FastQCFastQC Report
Tue 22 Apr 2025
Site1h_S94_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1h_S94_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73036
Total Bases12 Mbp
Sequences flagged as poor quality0
Sequence length40-281
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA61248.384906073717072No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG49236.740511528561258No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA26663.65025466893039No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG22443.0724574182594884No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG19942.7301604688099017No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG19882.721945342023112No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG18382.51656717235336No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA16702.286543622323238No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGG15032.057889260090914No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC13881.9004326633441042No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT13171.8032203297004217No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGT12531.7155923106413273No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG12441.7032696204611426No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG11301.547182211512131No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC10841.484199572813407No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG9811.3431732296401775No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA9721.3308505394599923No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA9381.2842981543348486No Hit
GAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT8831.2089928254559397No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG8331.1405334355660224No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC8231.1268415575880386No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA7411.0145681581685744No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA7331.0036146557861876No Hit
AAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT6810.9324168903006737No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATTCAGTTCGA6450.8831261295799331No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA6130.8393121200503861No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA4920.6736403965167862No Hit
GAACCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT4560.6243496357960457No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC4300.5887507530532887No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA4080.5586286215017252No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT4010.5490443069171368No Hit
AAACCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT3490.4778465414316228No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTCGA3300.4518319732734542No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG3190.43677090749767233No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG3070.42034065392409226No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA2480.3395585738539898No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2180.29848293992003944No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC2140.29300618872884604No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT1850.253299742592694No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC1800.24645380360370228No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAGATTTAT1710.23413111342351717No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC1700.23276192562571882No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA1650.2259159866367271No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1620.22180842324333203No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC1620.22180842324333203No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1590.217700859849937No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG1550.21222410865874364No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA1470.20127060627635687No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA1460.19990141847855852No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA1430.1957938550851635No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGA1370.1875787282983734No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1300.17799441371378497No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAACATTGC1280.1752560381181883No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA1250.17114847472479325No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1190.16293334793800318No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1180.16156416014020483No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1170.16019497234240648No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA1160.15882578454460813No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1150.1574565967468098No Hit
GAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT1120.15334903335341477No Hit
GAATCAAAATAAATGTTGGTATAAAACCGGATCTCCCCCTCCACTAGCGT1100.15061065775781807No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGACTCGA1090.14924146996001972No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGTCAAGTTCGA1090.14924146996001972No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG1040.142395530971028No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGG1020.1396571553754313No Hit
GAATCAAAACAAGTGTTGATATAAAATGGGGTCCCCTCCCCCTACAGGAT990.13554959198203625No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC960.1314420285886412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG960.1314420285886412No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT940.12870365299304454No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT930.1273344651952462No Hit
AAATCAAAATAAATGTTGGTATAAAACCGGATCTCCCCCTCCACTAGCGT910.12459608959964949No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT900.12322690180185114No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG890.12185771400405279No Hit
AAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT890.12185771400405279No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT860.11775015061065774No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG830.11364258721726272No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA830.11364258721726272No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG810.11090421162166601No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG780.106796648228271No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT760.10405827263267431No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCCACCAAATATTATTTGC750.10268908483487595No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGAGGGTTTAA740.1013198970370776No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAACGTTAAGGTTTGC740.1013198970370776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)