FastQCFastQC Report
Tue 22 Apr 2025
Site1h_S94_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1h_S94_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73036
Total Bases12 Mbp
Sequences flagged as poor quality0
Sequence length58-281
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG45746.262664987129635No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT45556.236650418971466No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT27853.8131880168683936No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT20242.7712361027438526No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT20012.7397447833944906No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGCCTGGCGGAT18402.519305547948957No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT16512.2605290541650693No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA14111.9319239826934662No Hit
CTACCGATTGGCTGTGGGATAGAGTTTGGGGGAGGGTGTCTGTTCTTGGC13411.8360808368475818No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT12181.6676707377183857No Hit
CTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGGCTTCGGGGAGCCG11411.5622432772879127No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG10101.3828796757763295No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT9901.3554959198203624No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG9851.3486499808313708No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG9281.270606276356865No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT7841.0734432334739032No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT7821.0707048578783065No Hit
TCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG7801.0679664822827097No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG6790.9296785147050769No Hit
TCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG6690.9159866367270935No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT6530.89407963196232No Hit
CTACCGATTGGATGATTTAGTGAGGATCTTGGACTGGCTGCTCGCGGGGT6480.8872336929733281No Hit
CCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG5700.7804370447450572No Hit
CCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG5260.72019278164193No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA4970.680486335505778No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT4530.6202420724026507No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA4490.6147653212114573No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT4160.569582123884112No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT4160.569582123884112No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT4130.5654745604907169No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG3970.5435675557259434No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT3930.53809080453475No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGAGGTT3860.5285064899501616No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG3730.5107070485787831No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT3710.5079686729831864No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT3530.48332329262281615No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG3450.47236979024042935No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG3410.46689303904923596No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT3270.4477244098800592No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT3010.4121255271373021No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG3000.4107563393395038No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCCTGGCGTGGTG2990.4093871515417054No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT2870.39295689796812533No Hit
CTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGGCGTTGAGAAGCCG2660.36420395421436No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG2640.3614655786187634No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT2350.32175913248261134No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG2320.31765156908921627No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGTACTGTGCTTGGTCTTG2220.30395969111123283No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2100.2875294375376527No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGATTGGACGTCGAGAGGTT2010.27520674735746753No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2000.2738375595596692No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC1800.24645380360370228No Hit
CTACCGATTGAATGGTTTGATGAATTGCGCGGACCATCCGCTTTGGAGGT1790.24508461580590393No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG1780.24371542800810558No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1710.23413111342351717No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC1600.21907004764773536No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1580.21633167205213866No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGACCGGCCCCTTCACGGCG1550.21222410865874364No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1540.2108549208609453No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1530.20948573306314697No Hit
CTACCGATTGGGTGTACCAGTGAGATCCTTGGACTGGCGAGCCTTGTGCG1460.19990141847855852No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCCTCCTTTGGACGG1440.19716304288296183No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTTAATGTGGTATTCGAGCC1440.19716304288296183No Hit
CTACCGATTGGCTGTGGGATAGAGCTTGGGAGAGGGAAGCTCGTTCTTGG1420.19442466728736513No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1310.17936360151158331No Hit
CATCTTAGTTGGGGATGGATGAGGCTGCGTTTTAATGGCGTATTCGAGTC1230.16841009912919655No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1160.15882578454460813No Hit
TCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA1130.1547182211512131No Hit
CTACCGATTGAGTGGTTTAGTGAGATCCCCGGATCGGCGCCGACATGGGG1110.15197984555561642No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG1080.14787228216222137No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG1060.14513390656662467No Hit
CTACCGATTGGCTGTGGGATAGAGCTGGACGGAGATGAGCGTTGAGGGCT1050.14376471876882635No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT1030.14102634317322962No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1030.14102634317322962No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1020.1396571553754313No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1010.13828796757763295No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1000.1369187797798346No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG990.13554959198203625No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGCGACGCCAGAGCGC980.1341804041842379No Hit
CTACCGATTGAATGGTTTAGTGAGTTCCTCGGATTGGTCCCGGCATGGTG940.12870365299304454No Hit
TCCCCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA880.12048852620625446No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGTGGCTTGTGCGCTT870.11911933840845612No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA850.11638096281285941No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT850.11638096281285941No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG840.11501177501506106No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC820.11227339941946438No Hit
CCCCCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA810.11090421162166601No Hit
CTACCGATTGAATGTGCTGGTGAAGACTTCGGATTGGCTGTTTGGTTAGG810.11090421162166601No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT800.10953502382386768No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA800.10953502382386768No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG800.10953502382386768No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG790.10816583602606933No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC780.106796648228271No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTTGGACTATGGTTTTCTGATGG780.106796648228271No Hit
CTAGCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG770.10542746043047264No Hit
CCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA770.10542746043047264No Hit
CTACCGATTGAACGTTCCGGTGAGACCTTTGGATCTGCTGATGTGGGGCG750.10268908483487595No Hit
CTACCGACTGCCAGGTTTTGTGAGCTATCTGGACCGCCAGTAGTGTGGGC740.1013198970370776No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)