FastQCFastQC Report
Tue 22 Apr 2025
Site1g_S93_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1g_S93_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206230
Total Bases35.2 Mbp
Sequences flagged as poor quality0
Sequence length36-281
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA203689.87635164622024No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG201349.762886098045872No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG107915.2325073946564515No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG89354.3325413373418025No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG85444.142947194879504No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG47452.300829171313582No Hit
GAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT34441.6699801192842942No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGA32681.5846385104010086No Hit
AAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT25231.2233913591621006No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGT24611.193327837850943No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA19130.9276051011007128No Hit
GAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT18460.895117102264462No Hit
GAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT17570.8519614023178005No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC17520.8495369247927073No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA17010.8248072540367551No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA16890.818988507976531No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA16480.7991077922707656No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC15640.7583765698491975No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG15490.7511031372739175No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA15400.7467390777287495No Hit
AAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT13130.6366677980895117No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA12460.6041797992532609No Hit
AAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT12440.6032100082432236No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG12270.5949667846579063No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT11820.5731464869320662No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11110.5387189060757407No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG10380.5033215342093779No Hit
GAACCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT10110.4902293555738738No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT8370.40585753770062555No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC7680.3723997478543374No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7570.3670658972991321No Hit
AAACCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT6990.33894195800804927No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA6660.3229404063424332No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6230.30208989962663046No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6230.30208989962663046No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT5630.27299616932551035No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA5270.2555399311448383No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4590.22256703680356882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4270.20705038064297143No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4180.20268632109780343No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT4100.19880715705765406No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA4020.1949279930175047No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA3810.1847451874121127No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA3800.18426029190709403No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3640.17650196382679534No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG3550.1721379042816273No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG3520.1706832177665713No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT3400.16486447170634727No Hit
GAATCAAAATAAATGTTGATACAAAACTGGATCACCTCCTCCGGCGGGGT3320.16098530766619792No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA3310.16050041216117927No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC3230.15662124812102993No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA3180.1541967705959366No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT3090.14983271105076856No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2940.14255927847548852No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2770.13431605489017118No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2660.12898220433496582No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAACATTGC2560.12413324928477913No Hit
CTACGGTTACCTTGTTACGACTTCACCCCAGTTACTGAATCCACTGTGGA2530.12267856276972312No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC2470.1197691897396111No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA2280.11055617514425642No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2260.10958638413421906No Hit
AAATCAAAATAAATGTTGATACAAAACTGGATCACCTCCTCCGGCGGGGT2220.1076468021141444No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2200.10667701110410707No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGTCAAGTTCGA2170.10522232458905105No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2140.10376763807399506No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATAAATAGGTTAAA2120.10279784706395771No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAACGTTAAGGTTTGC2110.10231295155893905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)