FastQCFastQC Report
Tue 22 Apr 2025
Site1f_S92_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1f_S92_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294887
Total Bases43.6 Mbp
Sequences flagged as poor quality0
Sequence length53-281
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG222667.550688907954573No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT201616.8368561516784405No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT184596.259685913587239No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT183616.226452844648967No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG117953.999837225784792No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGAATTAGGTTAGTT94023.188339940383943No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT93423.1679931634829614No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT79332.6901830192582246No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG68202.3127503077450005No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG64652.1923652110808547No Hit
CTACCGATTGGCTGTGGGATAGAGTTTGGGGGAGGGTGTCTGTTCTTGGC54361.8434179872290064No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT54211.8383312930037607No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG52401.7769518493524639No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG43631.4795497936497708No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT33991.1526449114406536No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT29470.9993658587865861No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG28850.9783408559889042No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTTGGGAGGAT24780.8403218860105736No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT22930.7775859905658777No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG20890.7084069491025375No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA20450.6934859793751504No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA19810.6717827506807692No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG19020.6449928277611424No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG17530.594464998457036No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGCGACGCCAGAGCGC15960.5412242655661321No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG14290.484592403191731No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG12470.42287384659208443No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA11970.4059181991745991No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT11410.3869278740670155No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT11210.3801456151000214No Hit
CTACCGATTGAATGGTTTAGTGAGGTCATCGGATTGGGCGCATGAGAGTG10980.37234601728797806No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT10720.36352908063088574No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC10210.3462343202650507No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG9940.3370782706596086No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG9500.3221573009322215No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT9410.31910528439707414No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT9340.3167314937586262No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA9250.31367947722347883No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGACCGGCCCCTTCACGGCG9110.30893189594658294No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA9060.3072363312048344No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC8900.3018105240312391No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT8760.2970629427543432No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA7980.27061213278306606No Hit
CTACCGATTGGGTGTACCAGTGAGAGCCTTGGATTGGCGGCCGTTGCCTT7900.2678992291962684No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA7390.25060446883043336No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC7140.2421266451216907No Hit
CTACTGATTGAGTGAACTGGTGAAATCCTTGGACAGCAGCACAGCCCAGA7050.23907462858654333No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT6810.23093591782615036No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG6800.23059680487780065No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG6740.2285621271877024No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG6430.2180496257888615No Hit
CTACCGATTGGCTGTGGGATAGAGCTTGGGAGAGGGAAGCTCGTTCTTGG6380.21635406104711297No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT6290.21330204451196563No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT6140.20821535028672No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG5850.19838107478457853No Hit
CTACCGATTGAGTGGTTTAGTGAGATCCCCGGATCGGCGCCGACATGGGG5660.19193792876593407No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC5350.18142542736709316No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT4870.16514800584630723No Hit
TTTCCGATGATGGTGGAATGCAGGTGATCGGACTGGCTTCGGCCGGAAAG4820.1634524411045587No Hit
CTACCGATTGAACGTTCCGGTGAGACCTTTGGATCTGCTGATGTGGGGCG4820.1634524411045587No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG4640.15734840803426398No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG4580.15531373034416573No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG4540.1539572785507669No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT4460.15124437496396925No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG4380.1485314713771716No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT4360.14785324548047218No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC4360.14785324548047218No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATAGATGGACTGTG4350.14751413253212248No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGTGAAGGGT4180.14174921241017746No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATGGATGGATTGTG4140.14039276061677863No Hit
CTACCGATTGAATGGTTTAGTGAGTTCCTCGGATTGGTCCCGGCATGGTG4010.13598429228823244No Hit
CTACCGATTGAATGGTTTAGTGAGACCCTCGGATTTGTCCCAGCAGGGTG4000.13564517933988274No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG3980.13496695344318332No Hit
CTACCGATTGAATGTGCTGGTGAAGACTTCGGATTGGCTGTTTGGTTAGG3950.1339496145981342No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT3900.13225404985638567No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG3820.12954114626958801No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT3780.1281846944761892No Hit
CTACCGATTGAACGTGCTGGTGAAGAGTTTGGATTGGAGTTTTGGCTAGG3720.12615001678609095No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG3700.12547179088939153No Hit
CTACCGATTGGATGGTCCGGTGAGAATTTCGGATTGTGGTTGATTTGAGT3650.123776226147643No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTTTTTACGCTT3530.11970687076744652No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT3500.1186895319223974No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGCGCAGG3440.11665485423229915No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT3440.11665485423229915No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG3370.1142810635938512No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT3280.11122904705870384No Hit
CTTCCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACCGAGAGCAGCCCCG3250.11021170821365472No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT3230.1095334823169553No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC3150.10682057873015766No Hit
CTACCGATTGAGTGGTCCGGTGAGGCTTTGGGAGAATTGGGTGGACTGTG3130.10614235283345824No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT3050.10342944924666057No Hit
GTTCCGATTTCGAGTGGTCCGGTGAACCTTTCGGACCGGGGAAGCCTTGA2990.10139477155656235No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGGAACTTGAGCGCTT2960.10037743271151323No Hit
CTACCGATTGAATGGTCCGGTGAAGTCTTCGGATTATGGTCTGCCTTGAG2960.10037743271151323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)