FastQCFastQC Report
Tue 22 Apr 2025
Site1ee_S142_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1ee_S142_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216951
Total Bases34.6 Mbp
Sequences flagged as poor quality0
Sequence length23-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG2228010.269600047937091No Hit
AAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG155297.157837484040175No Hit
GAACCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG125005.761669685781582No Hit
AAACCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG83483.8478734829523713No Hit
GAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAGGGGGG26031.1998100953671567No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT17260.795571350212721No Hit
AAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAGGGGGG16970.7822042765417077No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT15400.7098377052882909No Hit
GAACCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAGGGGGG14030.6466898055321249No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT13060.6019792487704597No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT13010.599674580896147No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT11970.5517374891104443No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT11470.5286908103673179No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT11330.5222377403192426No Hit
GAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT10850.5001129287258413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10510.4844411871805154No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10410.47983185143189017No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9210.42451982244838693No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT9010.41530115095113646No Hit
AAACCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAGGGGGG8750.4033168780047108No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT8440.38902793718397244No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT8380.3862623357347973No Hit
AAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT7550.3480048490212076No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT7200.33187217390101914No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7130.3286456388769814No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7090.32680190457753133No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT6930.3194269673797309No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT6500.29960682366064223No Hit
GAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT6330.2917709528879793No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT5960.27471641061806584No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5480.25259159902466455No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT4860.22401371738318793No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4730.2180215809099751No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4690.21617784661052494No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4670.21525597946079988No Hit
AAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT4210.19405303501712368No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT4190.19313116786739865No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT4150.19128743356794853No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT4050.18667809781932326No Hit
AATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCGA3990.1839124963701481No Hit
GAATCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT3820.17607662559748516No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3780.17423289129803504No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3730.1719282234237224No Hit
GAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT3710.17100635627399735No Hit
GAATCAGTTCCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG3500.1613267512018843No Hit
GAATCACGAGACGGAGGATAAACAGTTCAACCAGTACCCTGTCTCTTATA3490.16086581762702176No Hit
GAATCACGAGACGGAGGATAAACAGTTCAACCAGTTCCCTGTCTCTTATA3410.15717834902812156No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3390.15625648187839652No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT3270.1507252789800462No Hit
GAATCACGAGACGGAGGATAAACAGTTCAACCTGTACCCTGTCTCTTATA3170.14611594323142094No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT3080.1419675410576582No Hit
GAATCACGAGACGGGGGATAAACAGTTCAACCAGTACCCTGTCTCTTATA2980.13735820530903292No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2970.13689727173417038No Hit
GAATCACGAGACGGAGGATAAACTGTTCAACCTGTACCCTGTCTCTTATA2950.13597540458444535No Hit
AAATCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT2930.1350535374347203No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2860.1318270024106826No Hit
GAATCACGAGACGGAGGATAAACTGTTCAACCAGTACCCTGTCTCTTATA2860.1318270024106826No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2850.13136606883582005No Hit
GAATCACGAGACGGAGGATAAACAGTTCAACCTGTTCCCTGTCTCTTATA2830.13044420168609502No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT2820.12998326811123248No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2810.12952233453636997No Hit
AAATCACGAGACGGAGGATAAACAGTTCAACCAGTACCCTGTCTCTTATA2730.12583486593746976No Hit
GAATCACGAGACGGAGGATAAACAGTTCATCCAGTACCCTGTCTCTTATA2690.12399113163801964No Hit
GAATCACGAGACGGGGGATAAACAGTTCAACCAGTTCCCTGTCTCTTATA2620.12076459661398196No Hit
GAATCACGAGACGGAGGGTAAACAGTTCAACCAGTACCCTGTCTCTTATA2620.12076459661398196No Hit
GAATCACGAGACGGGGGATAAACAGTTCAACCTGTACCCTGTCTCTTATA2590.11938179588939439No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2570.11845992873966932No Hit
AAATCAGTTCCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG2540.11707712801508174No Hit
GAATCACGAGACGGGGGGTAAACAGTTCAACCAGTACCCTGTCTCTTATA2500.11523339371563165No Hit
GAATCACGAGACGGAGGATAAACAGTTCATCCAGTTCCCTGTCTCTTATA2490.11477246014076911No Hit
GAATCACGAGACGGAGGATAAACTGTTCAACCAGTTCCCTGTCTCTTATA2480.11431152656590658No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2460.11338965941618154No Hit
GAACCAGTTCCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCG2460.11338965941618154No Hit
GAATCACGAGACGGAGGATATACAGTTCAACCAGTACCCTGTCTCTTATA2450.11292872584131901No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2430.11200685869159396No Hit
AAATCACGAGACGGAGGATAAACAGTTCAACCTGTACCCTGTCTCTTATA2400.11062405796700638No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2370.1092412572424188No Hit
GAATCACGAGACGGAGGATATACAGTTCAACCAGTTCCCTGTCTCTTATA2350.10831939009269376No Hit
AAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT2350.10831939009269376No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2340.10785845651783121No Hit
GAATCACGAGACGGAGGATATACAGTTCAACCTGTACCCTGTCTCTTATA2300.10601472221838111No Hit
AAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT2280.10509285506865607No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT2280.10509285506865607No Hit
GAATCACGAGACGGGGGATAAACAGTTCAACCTGTTCCCTGTCTCTTATA2270.10463192149379352No Hit
GAATCACGAGACGGAGGGTAAACAGTTCAACCAGTTCCCTGTCTCTTATA2260.104170987918931No Hit
GAATCACGAGACGGAGGATATACTGTTCAACCAGTTCCCTGTCTCTTATA2250.10371005434406848No Hit
GAATCACGAGACGGAGGATATACAGTTCATCCAGTACCCTGTCTCTTATA2250.10371005434406848No Hit
GAATCACGAGACGGGGGATAAACAGTTCATCCAGTACCCTGTCTCTTATA2240.10324912076920595No Hit
GAATCACGAGACGGAGGATAAACAGTTCATCCTGTACCCTGTCTCTTATA2240.10324912076920595No Hit
GAATCACGAGACGGAGGATATACTGTTCAACCAGTACCCTGTCTCTTATA2230.10278818719434342No Hit
GAATCACGAGACGGGGGGTAAACAGTTCAACCAGTTCCCTGTCTCTTATA2200.10140538646975583No Hit
AACCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGAAATGCGA2190.10094445289489332No Hit
GAATCACGAGACGGAGGATAAACAGTTCATCCTGTTCCCTGTCTCTTATA2170.10002258574516826No Hit
GAATCACGAGACGGGGGGTAAACAGTTCAACCTGTACCCTGTCTCTTATA2170.10002258574516826No Hit
GAATCACGAGACGGGGGATATACAGTTCAACCAGTACCCTGTCTCTTATA2170.10002258574516826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)