FastQCFastQC Report
Tue 22 Apr 2025
Site1ee_S142_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1ee_S142_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216951
Total Bases33.8 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGT3227614.877092062262905No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGT2633112.136841959705187No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG23211.0698268272559242No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG18670.8605629842683371No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG16440.7577747970739936No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG15750.7259703804084794No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG14480.6674318164009384No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA14250.6568303441791004No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG13910.6411586026337744No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG11520.5309954782416306No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA11520.5309954782416306No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG11320.5217768067443801No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG11130.5130190688219921No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA10950.5047222644744667No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG10680.49227705795317833No Hit
TCCTCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA10520.4849021207553779No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG10420.4802927850067527No Hit
CCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA10140.46738664491060194No Hit
TCCTCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA9680.4461837004669257No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG9530.43926969684398787No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG9450.4355822282450876No Hit
CCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA9350.43097289249646237No Hit
TCCTCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA9090.41898861955003663No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8750.4033168780047108No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG8720.40193407728012315No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG8660.399168475830948No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA8630.3977856751063605No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA8540.3936372729325977No Hit
TCCCCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA8440.38902793718397244No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG8230.37934833211185937No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG8150.37566086351295913No Hit
TCCCCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA7820.36045005554249576No Hit
TCCTCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA7810.3599891219676332No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7650.3526141847698328No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG7370.3397080446736821No Hit
CCCTCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA7270.3350987089250568No Hit
CCCTCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA7210.33233310747588163No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7090.32680190457753133No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG7040.32449723670321867No Hit
TCCTCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA7040.32449723670321867No Hit
CCCCCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA7030.3240363031283562No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6900.31804416665514335No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA6880.31712229950541826No Hit
CCCCCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA6850.3157394987808307No Hit
TCCTCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA6720.30974736230761785No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA6620.3051380265589926No Hit
TCCCCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA6570.30283335868468No Hit
TCCTCCGTCTCGTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA6480.29868495651091725No Hit
TCCTCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA6430.2963802886366046No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG6320.2913100193131168No Hit
CCCTCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA6280.28946628501366667No Hit
TCCTCCGTCTCGTGATTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA6230.28716161713935406No Hit
TCCCCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA6020.277482012067241No Hit
CCCTCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA5960.27471641061806584No Hit
CCCCCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA5930.27333360989347827No Hit
TCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA5870.27056800844430307No Hit
CCCCCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA5840.2691852077197155No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG5800.26734147342026543No Hit
TCCCCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA5760.2654977391208153No Hit
CCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG5540.2553572004738397No Hit
TCCTCCGTCTCGTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA5500.2535134661743896No Hit
CCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA5400.24890413042576434No Hit
CCCTCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA5350.24659946255145168No Hit
CCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG5320.2452166618268641No Hit
CCCGAAGTCTACTGTCTCTTAACACATCTCCGAGCCCACGAGACGACGTG5270.2429119939525515No Hit
TCCCCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA5250.24199012680282644No Hit
CCCTCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA5220.24060732607823887No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA5150.23738079105420118No Hit
CCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA5130.23645892390447612No Hit
TCCCCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA5050.23277145530557591No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA5040.2323105217307134No Hit
TCCCCCGTCTCGTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA4980.22954492028153822No Hit
TCCTCCGTCTCGTGATTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA4920.22677931883236307No Hit
TCCTCCGTCTCGTGATTCTTTGGCCATCCCGAGGTCTACTGTCTCTTATA4900.22585745168263802No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG4810.22170904950887527No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4730.2180215809099751No Hit
CCCCCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA4610.21249037801162474No Hit
TCCTCCGTCTCGTGATTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA4520.208341975837862No Hit
TCCCCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA4500.20742010868813696No Hit
TCCTCCGTCTCGTGATTTTTTGGCCATCCCGAAGTCTACTGTCTCTTATA4490.2069591751132744No Hit
CCCCCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA4480.2064982415384119No Hit
TCCCCCGTCTCGTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA4410.20327170651437423No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG4370.2014279722149241No Hit
CCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA4360.20096703864006157No Hit
CCCTCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA4340.20004517149033652No Hit
TCCCCCGTCTCGTGATTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA4300.19820143719088643No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4270.19681863646629885No Hit
TCCTCCGTCTCGTGGTTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA4180.1926702342925361No Hit
CCCTCCGTCTCGTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA4170.19220930071767356No Hit
CCCTCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA4160.19174836714281107No Hit
CCCTCCGTCTCGTGATTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA4130.19036556641822347No Hit
CCCCCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA4090.18852183211877338No Hit
CCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA4030.1857562306695982No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA4020.18529529709473566No Hit
CCCGAGGTCTACTGTCTCTTAACACATCTCCGAGCCCACGAGACGACGTG3970.18299062922042306No Hit
CCCCCCGTCTCGTGATTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA3900.17976409419638537No Hit
TCCCCCGTCTCGTGATTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA3860.17792035989693525No Hit
CCCCCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA3860.17792035989693525No Hit
CCCTCCGTCTCGTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA3860.17792035989693525No Hit
CCCCCCGTCTCGTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA3850.17745942632207273No Hit
CCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG3850.17745942632207273No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG3820.17607662559748516No Hit
TCCCCCGTCTCGTGATTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA3810.17561569202262262No Hit
TCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA3790.17469382487289759No Hit
CCCTCCGTCTCGTGATTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA3660.16870168839968472No Hit
TCCTCCGTCTCGTGGTTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA3570.164553286225922No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA3550.1636314190761969No Hit
TCCTCCGTCTCGTGGTTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA3500.1613267512018843No Hit
CCCTCCGTCTCGTGGTTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA3440.15856114975270913No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3430.15810021617784661No Hit
TCCCCCGTCTCGTGATTCTTTGGCCATCCCGAGGTCTACTGTCTCTTATA3430.15810021617784661No Hit
TCCTCCGTCTCGTGGTTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA3410.15717834902812156No Hit
CCCCCCGTCTCGTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA3380.15579554830353398No Hit
TCCCCCGTCTCGTGGTTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA3260.15026434540518366No Hit
CCCTCCGTCTCGTGATTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA3250.14980341183032111No Hit
CCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA3240.14934247825545863No Hit
CCCTCCGTCTCGTGATTCTTTGGCCATCCCGAGGTCTACTGTCTCTTATA3230.14888154468059608No Hit
CCCCCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA3200.1474987439560085No Hit
CCCCCCGTCTCGTGATTCTTTGGCCATCCCGAGGTCTACTGTCTCTTATA3170.14611594323142094No Hit
CCCCCCGTCTCGTGGTTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA3110.14335034178224576No Hit
TCCTCCGTCTCGTGATTTTTTGGCCATCCCGAGGTCTACTGTCTCTTATA3070.14150660748279564No Hit
TCCCCCGTCTCGTGATTTTTTGGCCATCCCGAAGTCTACTGTCTCTTATA3050.1405847403330706No Hit
CCCCCCGTCTCGTGATTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA3000.13828007245875795No Hit
TCCCCCGTCTCGTGGTTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA2980.13735820530903292No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA2980.13735820530903292No Hit
TCCCCCGTCTCGTGGTTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA2980.13735820530903292No Hit
CCCCCCGTCTCGTGATTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA2970.13689727173417038No Hit
CCCTCCGTCTCGTGGTTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA2950.13597540458444535No Hit
CCCCCCGTCTCGTGATTTTTTGGCCATCCCGAAGTCTACTGTCTCTTATA2940.1355144710095828No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2940.1355144710095828No Hit
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG2880.13274886956040766No Hit
TCCCCCGTCTCGTGGTTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA2820.12998326811123248No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2750.1267567330871948No Hit
CCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG2750.1267567330871948No Hit
CCCTCCGTCTCGTGGTTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA2720.12537393236260722No Hit
TCCCCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG2710.1249129987877447No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2690.12399113163801964No Hit
TCCTCCGTCTCGTGGTTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA2670.12306926448829458No Hit
CCCTCCGTCTCGTGATTTTTTGGCCATCCCGAAGTCTACTGTCTCTTATA2640.12168646376370701No Hit
CCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA2640.12168646376370701No Hit
TCCCCCGTCTCGTGATTTTTTGGCCATCCCGAGGTCTACTGTCTCTTATA2630.12122553018884448No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2600.11984272946425689No Hit
TCCTCCGTCTCGTGGTTCTTTGGCCATCCCGAGGTCTACTGTCTCTTATA2580.11892086231453185No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG2580.11892086231453185No Hit
CCCCCCGTCTCGTGGTTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA2560.1179989951648068No Hit
TCCTCCGTCTCGTGGTTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA2550.11753806158994427No Hit
CCCTCCGTCTCGTGGTTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA2530.11661619444021923No Hit
TCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG2510.11569432729049416No Hit
CCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG2430.11200685869159396No Hit
CCCTCCGTCTCGTGGTTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA2430.11200685869159396No Hit
CCCCCCGTCTCGTGGTTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA2410.1110849915418689No Hit
TCCTCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG2370.1092412572424188No Hit
TCCTCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG2360.10878032366755626No Hit
TCCCCCGTCTCGTGGTTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA2340.10785845651783121No Hit
TCCTCCGTCTCGTGGTTTTTTGGCCATCCCGAAGTCTACTGTCTCTTATA2320.10693658936810616No Hit
CCCTCCGTCTCGTGGTTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA2290.10555378864351857No Hit
CCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG2260.104170987918931No Hit
CCCCCCGTCTCGTGGTTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA2230.10278818719434342No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2220.1023272536194809No Hit
CCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGTG2200.10140538646975583No Hit
TCCCCCGTCTCGTGGTTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA2180.10048351932003079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)