FastQCFastQC Report
Tue 22 Apr 2025
Site1e_S91_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1e_S91_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30672
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT18325.972874282733438No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT17985.862023995826813No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT16035.226264997391758No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG14334.672013562858633No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT13394.365545122587376No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG6382.080073030777256No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT6202.0213875847678664No Hit
TCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG4031.313901930099113No Hit
CTACCGATTGGCTGTGGGATAGAGTTTGGGGGAGGGTGTCTGTTCTTGGC3601.1737089201877933No Hit
TCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG3521.1476264997391759No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG3391.1052425665101722No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG2970.9683098591549295No Hit
CCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG2800.9128847157016171No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG2690.8770213875847678No Hit
CCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG2580.8411580594679187No Hit
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACACGTT2210.7205268648930621No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG2210.7205268648930621No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1670.5444705268648931No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG1640.5346896191966615No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACACGTT1610.5249087115284298No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1540.5020865936358895No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1480.4825247782994262No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1420.4629629629629629No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1320.4303599374021909No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT1300.4238393322900365No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1300.4238393322900365No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT1230.40101721439749605No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1210.3944966092853417No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1170.3814553990610329No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG1160.37819509650495564No Hit
TCCTCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1130.36841418883672405No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA1110.36189358372456965No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG1090.35537297861241524No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG1070.3488523735002608No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGCGACGCCAGAGCGC1050.3423317683881064No Hit
CCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1030.335811163275952No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1030.335811163275952No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG1020.3325508607198748No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1010.3292905581637976No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG970.3162493479394888No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA930.30320813771517996No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG930.30320813771517996No Hit
CCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG900.2934272300469483No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT880.28690662493479396No Hit
CCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA860.28038601982263955No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG860.28038601982263955No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA850.2771257172665623No Hit
TCCTCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA820.26734480959833073No Hit
TCCTCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA800.2608242044861763No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT780.2543035993740219No Hit
CCCTCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA760.2477829942618675No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG730.23800208659363586No Hit
CCCCCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA730.23800208659363586No Hit
CCCTCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA710.23148148148148145No Hit
TCCTCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA710.23148148148148145No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT700.22822117892540428No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG670.21844027125717266No Hit
CCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG660.21517996870109546No Hit
TCCTCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA630.20539906103286387No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA630.20539906103286387No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT620.20213875847678664No Hit
TCCCCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA610.1988784559207094No Hit
CCCCCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA610.1988784559207094No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG610.1988784559207094No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG610.1988784559207094No Hit
CCCTCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA600.19561815336463223No Hit
TCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA600.19561815336463223No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG590.19235785080855505No Hit
TCCCCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA590.19235785080855505No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG590.19235785080855505No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGTATGCTGAG590.19235785080855505No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA590.19235785080855505No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA580.18909754825247782No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT580.18909754825247782No Hit
CCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA570.18583724569640062No Hit
TCCTCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA570.18583724569640062No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG570.18583724569640062No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG540.17605633802816903No Hit
CCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA530.17279603547209182No Hit
CCCCCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA510.1662754303599374No Hit
TCCCCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA510.1662754303599374No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG500.16301512780386018No Hit
TCCTCCGTCTCGTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA500.16301512780386018No Hit
CCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA500.16301512780386018No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG490.159754825247783No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA490.159754825247783No Hit
CTACCGATTGAGTGGTTTAGTGAGATCCCCGGATCGGCGCCGACATGGGG480.1564945226917058No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA470.1532342201356286No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGACCGGCCCCTTCACGGCG470.1532342201356286No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT450.14671361502347416No Hit
TCCCCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA450.14671361502347416No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA450.14671361502347416No Hit
TCCCCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA450.14671361502347416No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC440.14345331246739698No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG440.14345331246739698No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT440.14345331246739698No Hit
TCCCCCGTCTCGTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA440.14345331246739698No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG430.14019300991131978No Hit
TCCTCCGTCTCGTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA430.14019300991131978No Hit
TCCTCCGTCTCGTGATTTTTTGGCCACCCCGAGGTCTACTGTCTCTTATA420.13693270735524257No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA420.13693270735524257No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT420.13693270735524257No Hit
TCCCCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA420.13693270735524257No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT420.13693270735524257No Hit
CCCCCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA420.13693270735524257No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG410.13367240479916537No Hit
TCCTCCGTCTCGTGGTTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA410.13367240479916537No Hit
TCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA410.13367240479916537No Hit
CCCTCCGTCTCGTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATA400.13041210224308816No Hit
CCCCCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA400.13041210224308816No Hit
TCCTCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA390.12715179968701096No Hit
CCCCCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA390.12715179968701096No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC390.12715179968701096No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATAGATGGACTGTG390.12715179968701096No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA380.12389149713093375No Hit
TCCCCCGTCTCGTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATA380.12389149713093375No Hit
TCCCCCGTCTCGTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATA380.12389149713093375No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT380.12389149713093375No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG370.12063119457485655No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG370.12063119457485655No Hit
CCCTCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA370.12063119457485655No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG360.11737089201877934No Hit
TCCCCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA360.11737089201877934No Hit
CCCTCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA360.11737089201877934No Hit
CCCTCCGTCTCGTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATA360.11737089201877934No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG360.11737089201877934No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT360.11737089201877934No Hit
CTACCGATTGGCTGTGGGATAGAGCTTGGGAGAGGGAAGCTCGTTCTTGG350.11411058946270214No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG350.11411058946270214No Hit
TCCTCCGTCTCGTGATTCTTTGGCCATCCCGAGGTCTACTGTCTCTTATA350.11411058946270214No Hit
CTACCGATTGAGTGGTCCGGTGAACTTTTTGGACGTTTACTTGTTGAGCG340.11085028690662495No Hit
CCCCCCGTCTCGTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATA340.11085028690662495No Hit
CCCTCCGTCTCGTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATA330.10758998435054773No Hit
TCCTCCGTCTCGTGATTCTTTGGCCATCCCGAAGTCTACTGTCTCTTATA330.10758998435054773No Hit
CCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA320.10432968179447052No Hit
CCCCCCGTCTCGTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA320.10432968179447052No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT320.10432968179447052No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG320.10432968179447052No Hit
CCCTCCGTCTCGTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATA320.10432968179447052No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT310.10106937923839332No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG310.10106937923839332No Hit
TCCCCCGTCTCGTGATTTTTCGGCCATCCCGAGGTCTACTGTCTCTTATA310.10106937923839332No Hit
CTACCGATTGAATAGTTTAGTGAGGCACTTGGACTGGAACTCTCGAGGTT310.10106937923839332No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA310.10106937923839332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)