FastQCFastQC Report
Tue 22 Apr 2025
Site1dd_S141_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1dd_S141_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321229
Total Bases57.7 Mbp
Sequences flagged as poor quality0
Sequence length40-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG193496.023428768884503No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG184625.747301769142886No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG164805.130296455176837No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA131244.0855589003483495No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC114553.565991862503074No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC95092.960193506812897No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG78302.4375134250021016No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA77852.423504727157262No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA71982.240769046381242No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA57961.8043202824153486No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG57021.7750576691394613No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA49531.5418906761220188No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG41651.2965828116390488No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT38651.203191492673451No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGACAAGTTCGA35441.1032627813802613No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG33471.0419358152595188No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT32441.0098714624146636No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT28610.8906418785352507No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT27490.8557757861214275No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC26200.8156175189662205No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC26150.8140609969834603No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC22440.6985670658626711No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA22190.6907844559488714No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT22080.6873601075867994No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA21240.6612105382764322No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA21150.6584087987074642No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT20200.6288348810350248No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA19200.5977044413798256No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA19030.5924122666384418No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA18720.5827618303453299No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTTAC15630.48656877181076424No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT15360.4781635531038605No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT15100.47006963879350866No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT14090.43862789474175745No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGA13380.416525282586566No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT10980.34181222741408773No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT10970.34150092301753576No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10780.3355861394830479No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG10640.33122787793132No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG10510.32718092077614414No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC10170.31659657129337637No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG9740.3032104822416407No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC9250.28795656681059306No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT9150.28484352284507314No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT8820.27457047775885735No Hit
CTACGGCTACCTTGTTACGACTTCACCCCAGTCACTGACCCTACCGTGGT8520.26523134586229763No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA8490.26429743267264166No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT8120.25277917000021793No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG8030.24997743043124998No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG7960.24779829965538602No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGAGGTTCAC7940.24717569086228205No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT7630.23752525456917029No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA7560.23534612379330633No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA7550.23503481939675433No Hit
GAATCAAAATAGGTGCTGGTACAACACAGGGTCTCCCCCGCCGGCTACAT7240.22538438310364256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7190.2238278611208826No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6850.21324351163811484No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT6790.21137568525880293No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG6750.21013046767259497No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA6690.208262641293283No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA6590.20514959732776308No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT6260.19487655224154732No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6050.18833915991395547No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT6050.18833915991395547No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5950.18522611594843555No Hit
GAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT5690.17713220163808374No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG5570.17339654887945982No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5460.1699722005173879No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5220.1625008950001401No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5150.16032176422427616No Hit
AAATCAAAATAGGTGCTGGTACAACACAGGGTCTCCCCCGCCGGCTACAT5080.1581426334484122No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC4900.15253915431047632No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC4880.15191654551737235No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4830.1503600235346124No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG4780.1488035015518524No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA4710.14662437077598847No Hit
CTACAGAAGCCTTGTTACGACTTCTCCTTCCTCTGAGTGATAAGGTTCAC4680.14569045758633248No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT4530.1410208916380526No Hit
AAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT4520.1407095872415006No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT4500.14008697844839663No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4470.13915306525874063No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4400.1369739344828767No Hit
GAATCAGTACCCTATCTCTTATACACATCTGACGCTGCCGACGAGAGCAC4350.13541741250011674No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG4320.13448349931046077No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGC4290.13354958612080478No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4240.13199306413804482No Hit
CTACAGAAGGACGGGCGGTGTGTACCTGTCTCTTATACACATCTGACGCT4230.13168175974149285No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC4210.13105915094838885No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4160.1295026289656289No Hit
GAACCAAAATAGGTGCTGGTACAACACAGGGTCTCCCCCGCCGGCTACAT4140.1288800201725249No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT4130.1285687157759729No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA4090.12732349818976493No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA4010.12483306301734899No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT3930.12234262784493305No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA3910.12172001905182907No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT3850.11985219267251711No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC3850.11985219267251711No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3790.11798436629320516No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT3580.11144697396561332No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA3570.11113566956906132No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3560.11082436517250932No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATCAGGTTTAC3560.11082436517250932No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC3540.11020175637940534No Hit
GAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT3460.1077113212069894No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3450.1074000168104374No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA3430.10677740801733342No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTCTAG3420.10646610362078143No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3390.10553219043112547No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA3340.1039756684483655No Hit
GAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT3310.10304175525870952No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG3280.10210784206905354No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA3260.10148523327594956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)