FastQCFastQC Report
Tue 22 Apr 2025
Site1d_S86_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1d_S86_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175036
Total Bases33 Mbp
Sequences flagged as poor quality0
Sequence length45-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG166739.525469046367604No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG104065.945062729952696No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG92035.257775543316804No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG74824.274549235585822No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG67393.850065129459083No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC61503.5135629241984505No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC48452.7680020110148766No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA38062.174409835690944No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT37312.13156150734706No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG27831.5899586370803722No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT25451.4539866084691149No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT20341.1620466646861216No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA20111.1489065106606642No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA16580.9472337119221189No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT13660.7804108869032657No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT13570.7752690875019996No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG12340.7049978290180307No Hit
GAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT11230.6415823030690829No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT10060.5747389108526245No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA9810.5604561347379968No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT9750.557028268470486No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG9680.5530290911583903No Hit
CTACGGAAACCTTGTTACGACTTTACCTTCCTCTAAATAACGAGGTTTAA9120.5210356726616239No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA8850.5056102744578258No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA8830.5044676523686555No Hit
AAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT8430.48161521058525103No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8320.4753307890948148No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA8090.46219063506935715No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA8060.46047670193560186No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA8020.45819145775726133No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC7770.44390868164263353No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG7670.4381955711967824No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC7130.40734477478918624No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG7010.4004890422541649No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6770.3867775771841221No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT6470.3696382458465687No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT6450.36849562375739847No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC6430.36735300166822826No Hit
GAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT6310.3604972691332069No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6090.3479284261523344No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC5950.3399300715281428No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG5610.3205054960122489No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC5570.31822025183390845No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTAG5540.3165063187001531No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC5520.3153636966109829No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA5250.2999382984071848No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA5140.29365387691674855No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5090.290797321693823No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT5060.28908338856006766No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5040.2879407664708974No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4900.2799424118467058No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4760.27194405722251425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.26166045841998214No Hit
AAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT4530.25880390319705665No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT4460.2548047258849608No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4020.2296670399232158No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT4020.2296670399232158No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCGATCAAGTTTGA3960.2262391736557051No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3940.22509655156653488No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3710.21195639754107726No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3660.20909984231815168No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT3570.20395804291688568No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA3550.20281542082771545No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA3210.18339084531182157No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC3130.17882035695514067No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT3080.1759638017322151No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2970.16967938024177884No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG2880.16453758084051281No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT2860.16339495875134258No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG2810.16053840352841703No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2780.15882447039466166No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA2760.15768184830549145No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2720.15539660412715098No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC2650.1513974268150552No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG2630.15025480472588496No Hit
CTACAGAAGCCTTGTTACGACTTCTCCTTCCTCTGAGTGATAAGGTTCAC2630.15025480472588496No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT2620.14968349368129985No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2600.14854087159212961No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT2570.14682693845837427No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2530.14454169428003383No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2480.14168513905710825No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTTAATAAATAGATTTAC2470.14111382801252315No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAATGTTTAG2330.13311547338833155No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2270.12968760712082086No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT2260.12911629607623576No Hit
GAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT2250.12854498503165063No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2080.1188326972737037No Hit
AAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT2030.11597614205077812No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA2020.115404831006193No Hit
CTACGGAAACCTTGTTATGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC2010.1148335199616079No Hit
AAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT1900.10854909847117164No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT1870.10683516533741631No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG1870.10683516533741631No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG1850.10569254324824606No Hit
AAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT1810.10340729906990562No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA1800.10283598802532051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)