FastQCFastQC Report
Tue 22 Apr 2025
Site1d_S86_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1d_S86_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175036
Total Bases33 Mbp
Sequences flagged as poor quality0
Sequence length54-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT170889.762563129870426No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT105886.049041340067187No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT94255.384606595214699No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT76404.36481638063027No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT39072.23211225119404No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG31181.781347837016385No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT27601.5768184830549143No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG27171.5522521081377545No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG26431.5099750908384562No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG21241.213464658698782No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG20271.1580474873740259No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG18991.0849196736671312No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG16500.9426632235654381No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT16160.923238648049544No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT15000.8569665668776708No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA13990.7992641513745744No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC13770.7866953083937018No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG13150.7512740236294249No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG11030.6301560821773806No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA10660.6090175735277314No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT10390.5935921753239334No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT10160.5804520212984757No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG9950.5684544893621883No Hit
CTACCGATTGAATGTCTCGGTGAAGACTTGGGATCGTAGCTCTGTTGCTT9880.5644553120500926No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG9460.5404602481775178No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG8700.4970406087890491No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG8480.48447176580817664No Hit
CTACCGATTGAGTGGTTCGGTGAACTTTTTGGACTGCGCAGGCTTTAGTC8470.4839004547635915No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG8440.48218652162983616No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG8030.45876276880184647No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG7930.4530496583559953No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG7720.441052126419708No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG7680.4387668822413675No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT7500.4284832834388354No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG7110.40620215270001603No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG6980.3987751091204095No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTTATGAGTTTCCTT6920.39534724285289885No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG6900.3942046207637286No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC6810.3890628213624626No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT6530.37306611211407936No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG6300.35992595808862177No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT6230.35592678077652595No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6130.3502136703306748No Hit
CCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG6000.34278662675106836No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA5930.33878744943897254No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT5920.3382161383943874No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG5820.3325030279485363No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG5660.3233620512351745No Hit
CTACCGATTGAATGGTCCGGTGAAATGTTCGGACCGCCGCGAAACGGACG5630.32164811810141913No Hit
CCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG5620.32107680705683406No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT5620.32107680705683406No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5480.31307845243264243No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA5470.3125071413880573No Hit
CTACCGATTGGATGGTCCGGTGAAGCCTCGGGATTGTGACCGGTGCCTTT5460.31193583034347216No Hit
CTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTC5440.31079320825430196No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5430.3102218972097169No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5320.3039374757192806No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA5300.3027948536301104No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG5280.3016522315409402No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5270.30108092049635504No Hit
CTACCGATTGAACGGTTTAGTGAGACCCTCGGATTGGTCCCGGTACGGGG4990.28508421124797184No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT4870.2782284787129505No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG4840.27651454557919514No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT4460.2548047258849608No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC4430.2530907927512055No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG4360.24909161543910968No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG4290.2450924381270139No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT4260.24337850499325853No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG4040.230809662012386No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT3980.22738179574487535No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC3960.2262391736557051No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3950.22566786261112No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG3860.220526063209854No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA3840.21938344112068375No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT3820.2182408190315135No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3790.2165268858977582No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG3760.21481295276400283No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATCGGCGCTCTGAAGGTT3570.20395804291688568No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCGAGGTGCCCGGTT3450.1971023103818643No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG3390.1936744441143536No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT3250.18567608949016204No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG3130.17882035695514067No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG3060.17482117964304486No Hit
CTACCGATTCAATGAATCGATGAACTGGATGGAGTGGCCTTATTAGGGGC2980.17025069128636394No Hit
CTACTGATTGAGTGAACTGGTGAAATCCTTGGACAGCAGCACAGCCCAGA2930.1673941360634384No Hit
CCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2920.16682282501885326No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2850.16282364770675745No Hit
CCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2820.16110971457300213No Hit
CATCTTAGTTGTTGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC2590.1479695605475445No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG2580.14739824950295938No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCGGTGCCTTA2510.1433990721908636No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC2460.140542516967938No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2420.13825727278959757No Hit
CTACCGATTGAATGATCCGGTGAATAACTCGGACTGATGCAGCGATCAGC2360.1348294065220869No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2310.1319728512991613No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2300.1314015402545762No Hit
CCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2220.12683105189789529No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT2210.12625974085331018No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT2210.12625974085331018No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC2180.12454580771955484No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2140.12226056354121438No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG2120.12111794145204416No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2060.11769007518453348No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2050.11711876413994836No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2030.11597614205077812No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT2010.1148335199616079No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG1990.11369089787243768No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACTGTGCGCGGCACTCGG1940.1108343426495121No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG1910.10912040951575674No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG1830.10454992115907584No Hit
TCCTCCATTATCAAGTGCAAACGCTCATTCAGGACCTTCAGTAGATTTAG1780.10169336593615028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)