FastQCFastQC Report
Tue 22 Apr 2025
Site1cc_S140_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1cc_S140_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354012
Total Bases60.3 Mbp
Sequences flagged as poor quality0
Sequence length50-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT258387.298622645560037No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT237266.702032699456516No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT101922.8789984520298746No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT96232.7182694371942193No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA70361.9875032484774526No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG69841.9728144808650556No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG66501.8784673965854266No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT52241.4756561924454539No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT45561.286962023886196No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG43951.241483339547812No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC42171.191202558105375No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT38331.0827316588138256No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT35220.99488152943968No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGAGGTT34180.9655039942148854No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT33840.9558998000067794No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG33830.9556173237065411No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT33770.9539224659051107No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG31720.8960148243562366No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG30390.858445476424528No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG30160.8519485215190445No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT28400.8022326926770844No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG26330.7437600985277335No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG24160.6824627413759985No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA23540.6649492107612172No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC23390.660712066257641No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT22990.6494130142481046No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA22770.6431985356428596No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG22680.6406562489407139No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG22650.6398088200399986No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG20960.5920703252997073No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG20620.5824661310916014No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA19300.5451792594601313No Hit
CTACCGATTGAATGATCCGATGAATATCGTGGACTGAGGGTGTAAGCGGG18680.5276657288453499No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG18060.5101521982305685No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG17370.49066133351411817No Hit
TCCTCCACTTGCCGGAAATCTTGCCCATGCAGGTGCATCTGTAGATTTAA16840.47569008960148246No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT15810.4465950306769262No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG15770.4454651254759726No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG14900.42088968735523086No Hit
TCCCCCACTTGCCGGAAATCTTGCCCATGCAGGTGCATCTGTAGATTTAA14710.4155226376507011No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG14150.39970396483735016No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA14110.39857405963639647No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG13490.3810605290216151No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG13230.3737161452154164No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC13200.3728687163147012No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG13050.36863157181112505No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG12720.3593098539032575No Hit
CCCTCCACTTGCCGGAAATCTTGCCCATGCAGGTGCATCTGTAGATTTAA12510.3533778515982509No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG12450.35168299379682044No Hit
CCCCCCACTTGCCGGAAATCTTGCCCATGCAGGTGCATCTGTAGATTTAA11890.33586432098346947No Hit
CTACCGATTGAACGTGCTGGTGAAGACTTCGGATTGGAAGCATGGCTAGG11850.3347344157825159No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG11840.33445193948227747No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA11250.31778583776821123No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG10650.3008372597539066No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA10140.28643096844174776No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG10090.2850185869405557No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG10070.28445363434007886No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG10020.2830412528388868No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT9940.28078144243697956No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT9850.2782391557348338No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC9790.2765442979334034No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG9720.2745669638317345No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT9360.26439781702315174No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG8820.2491440968102776No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG8470.23925742630193325No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG8380.23671513959978757No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG8240.23276047139644981No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT8110.2290882794933505No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTTAATGTGGTATTCGAGCC7870.22230884828762865No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCAGGAATCGCATGAGT7740.21863665638452934No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG7560.21355208298023798No Hit
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGTA7530.21270465407952271No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG7340.20733760437499296No Hit
CTACCGATCAGATGATTTAGTGAGGCCCTCGGATTGGTCCAGACTCAGGG7290.20592522287380088No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT7150.20197055467046313No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT7030.19858083906760224No Hit
CTTCCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACCGAGAGCAGCCCCG6970.19688598126617177No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6930.1957560760652181No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG6720.18982407376021151No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT6590.18615188185711218No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG6570.18558692925663536No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6480.18304464255448968No Hit
CTACCGATTGAATGATCCGGTGAGCTATTCGGACTTTCGCTTTGTCGATT6370.17993740325186716No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT6330.17880749805091353No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGTA6320.17852502175067514No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT6290.17767759284995988No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT6110.1725930194456685No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG6090.1720280668451917No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT5760.16270634893732416No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCTCGTGGCTTCG5620.15875168073398643No Hit
CTACCGATTGAATGGCTTAGTGAGCTCGAGGGACTGTTGCCGCTTGAGGG5490.1550794888308871No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT5380.1519722495282646No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT5340.15084234432731095No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC5310.1499949154265957No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC5240.14801758132492682No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGTATGCTGAG5170.14604024722325795No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG5160.14575777092301956No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTTGGACTATGGTTTTCTGACGG5090.1437804368213507No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG5090.1437804368213507No Hit
CTACCGATTGAGCAATCCGGTGAATATTTCGGACCGGACCCTGTCGGGGC5020.1418031027196818No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCTCCTT4850.13700100561562883No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG4780.13502367151395997No Hit
CTACCGATTGAGTGATCCGGTGAGTAATTCGGACCAGCTGTCGCCTGAGT4740.13389376631300634No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT4590.1296566218094302No Hit
CATGGAAGTGGATTGCACCAGAAGTAGATAGTCTAACCTTAGGGAGGGCG4560.12880919290871495No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT4510.12739681140752293No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCGGTGCCTTA4500.1271143351072845No Hit
CTACCGATTGAGCATTAGGGTGAAATCTTCGGACCGTGGCATACTTCTGG4470.12626690620656927No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT4440.12541947730585404No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4430.12513700100561564No Hit
CTACCGATTGAGTGATCCGGTGAATTGTTCGGACTGCGGCCATTTTCGGT4210.11892252240037061No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4180.11807509349965539No Hit
CTACCGATTGAACGGCTTAGTGAGTTTCGAGGATTTGGGTACTGTTCGGT4160.11751014089917855No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT4140.11694518829870175No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC4130.11666271199846333No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG4120.11638023569822491No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA4120.11638023569822491No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT4120.11638023569822491No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4090.11553280679750969No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGATCTTGGGAGGTT4040.11412042529631765No Hit
CTACCGATTCAATGAATCGATGAACTGGATGGAGTGGCCTTATTAGGGGC3970.11214309119464876No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT3940.11129566229393353No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG3930.11101318599369514No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG3920.11073070969345672No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG3830.10818842299131103No Hit
CTACCGATTGAATGGTCCGGTGAAATGTTCGGACTGTTGCGTGGCGAACG3750.10592861258940375No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3700.1045162310882117No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGATCTTGGGAGGGC3690.10423375478797328No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG3660.10338632588725806No Hit
CTACCGATTGAATGGTTTAGTGAGACCCTCGGACTGGAGTCCGTCTGCCT3620.10225642068630442No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCGAGGTGCCCGGTT3580.10112651548535077No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCAGAGCTTCG3580.10112651548535077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)