FastQCFastQC Report
Tue 22 Apr 2025
Site1c_S85_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1c_S85_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128106
Total Bases23.3 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG114258.91839570355799No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG70465.500132702605655No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC47373.6977190763898644No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC44383.464318611150141No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG40993.199694003403432No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG36212.826565500444944No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA33392.60643529577069No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTCGG25912.022543830890044No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT24151.885157603859304No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG23471.832076561597427No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA23171.8086584547171873No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA21101.6470735172435327No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG19931.5557429004105976No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA16611.2965825176026105No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT15361.1990070722682777No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG14961.1677829297612914No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC14131.1029928340592945No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA13631.0639626559255617No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT12300.9601423820898319No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT12130.9468721215243626No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA9620.7509406272930229No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA9220.7197164847860366No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG8860.6916147565297488No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC8800.6869311351537009No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGG8740.6822475137776529No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8730.6814669102149782No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT8710.6799057030896288No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG8030.626824660827752No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTAAGGTTCAC7460.5823302577552963No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC7330.5721824114405258No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA7310.5706212043151765No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7000.546422493872262No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG6380.49802507298643306No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5880.45899489485270006No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5660.4418216164738576No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5280.41215868109222054No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5220.4074750597161726No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5190.40513324902814857No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAATGATATGGTTTAA4910.3832763492732581No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA4850.3785927278972101No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT4780.3731285029584875No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4230.3301953070113812No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC3970.30989961438184005No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3960.3091190108191654No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3880.30287418231776814No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGC3790.29584875025369617No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3600.2810172825628776No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3190.24901253649321653No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG3040.23730348305309665No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA3040.23730348305309665No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACATCCAAGTTTGC2900.22637503317565144No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2850.22247201536227812No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2850.22247201536227812No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA2760.21544658329820618No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC2750.2146659797355315No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2750.2146659797355315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.2131047726101822No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2610.2037375298580863No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCATCTAAATGATAAGGTTTAG2560.19983451204471295No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA2490.19437028710599036No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2490.19437028710599036No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2360.18422244079121977No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2280.17797761228982248No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA2150.1678297659750519No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2130.1662685588497026No Hit
GAATCAGAATAAGTGTTGATAAAGAATAGGATCACCTCCTCCTGTAGGGT2080.1623655410363293No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC2040.15924312678563063No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG2020.15768191966028133No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2020.15768191966028133No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT2000.156120712534932No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT1970.15377890184690804No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1790.13972803771876416No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT1720.13426381278004154No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT1670.13036079496666825No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT1670.13036079496666825No Hit
AAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT1610.12567717359062025No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC1590.12411596646527095No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA1590.12411596646527095No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA1550.12099355221457231No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA1530.119432345089223No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG1510.11787113796387368No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC1480.11552932727584969No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC1440.11240691302515105No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1430.1116263094624764No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA1430.1116263094624764No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT1380.10772329164910309No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGAGGTTCAC1330.10382027383572978No Hit
AAATCAGAATAAGTGTTGATAAAGAATAGGATCACCTCCTCCTGTAGGGT1320.10303967027305513No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC1300.10147846314770581No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA1300.10147846314770581No Hit
GAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT1290.10069785958503115No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG1290.10069785958503115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)