FastQCFastQC Report
Tue 22 Apr 2025
Site1c_S85_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1c_S85_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128106
Total Bases23.3 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT115779.03704744508454No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT70935.536821070051364No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT35792.7937801508126086No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT26302.052987369834356No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT25982.0280080558287668No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG21331.6650273991850497No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT19611.5307635864050084No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG18901.4753407334551076No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG17921.3988415843129909No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG16401.2801898427864424No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG15331.1966652615802538No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT15231.1888592259535071No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG14401.1240691302515105No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG13741.0725492951149829No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT13381.0444475668586952No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG13291.0374221347946233No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG12370.9656066070285544No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA12330.9624841927778559No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA12060.94140789658564No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT10530.8219755514964171No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG10450.8157307229950197No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG10330.8063634802429238No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA9110.7111298455966153No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG9000.702543206407194No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG8680.6775638924016049No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG8500.663513028273461No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA8450.6596100104600877No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG8290.6471203534572932No Hit
CTACCGATTGAATAATCCGGTGAGGCCCCCGGACTGTGGCGTTGCTCCTG7990.6237022465770534No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC7880.6151156073876322No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC7680.5995035361341389No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA7630.5956005183207655No Hit
CTACCAATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT7400.5776466363792484No Hit
CTACCGATTGAGTGTTCCGGTGAATAATTCGGACTGCGTTAACGCTCAGT7390.5768660328165738No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT7220.5635957722511046No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATCGGCGCTCTGGAGGCT6870.5362746475574914No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG6660.5198819727413236No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAATGCTTGGA6630.5175401620532996No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG6570.5128565406772516No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT6500.507392315738529No Hit
CTACCGATTGAATGGTTTAGTGAGGTCTCGAGATTGGCGCTTTGGAGCTG6490.5066117121758543No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG6200.48397420885828923No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5890.4597754984153748No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG5880.45899489485270006No Hit
CTACCGATTGAATGGTCCGGTGAAATGTTCGGACCGCGGCCTCATTGCGG5530.431673770159087No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT5430.4238677345323404No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5330.41606169890559375No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT5310.4145004917802445No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT5220.4074750597161726No Hit
CTACCGATTGAATGGTTCGGTGAACCTTTTGGACTGCTTAGGATTTATTC5180.4043526454654739No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGGCCTGTCTTGGTT4820.37625091720918613No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4790.37390910652116216No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA4630.3614194495183676No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4550.35517462101697034No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT4260.3325371176994052No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4180.3262922891980079No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4030.314583235757888No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3920.3059965965684667No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3860.3013129751924188No Hit
CTACCGATTGAACGGCTTAGTGAGTTTCGAGGATCGGGGGTATCCAGAGG3570.27867547187485364No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3530.275553057624155No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG3490.27243064337345635No Hit
CTACCGATTGAACATTTTAGTGAGTTGCGAGGAGTGTCAGTTTTAGGGTT3190.24901253649321653No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3130.2443289151171686No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3120.24354831155449394No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2940.22949744742635003No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACGTCGAGAGGTT2860.2232526189249528No Hit
CTACCGATTGAATGATTTAGTGAGGCCTTCGGACTGGCGCTCTTGGATGC2850.22247201536227812No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT2720.21232416904750753No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2720.21232416904750753No Hit
CTACCAATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT2530.19749270135668898No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2510.19593149423133968No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCGGTGCCTTA2470.19280907998064103No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2370.18500304435389445No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC2340.18266123366587045No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2310.18031942297784648No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2290.17875821585249715No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2240.17485519803912386No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCTACCTTTGGCCTT2150.1678297659750519No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2150.1678297659750519No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT2140.16704916241237724No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC2110.16470735172435327No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC2040.15924312678563063No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2010.15690131609760666No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG1940.15143709115888407No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT1930.1506564875962094No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC1900.1483146769081854No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1830.1428504519694628No Hit
TCCTCCTTTATCAAGAAATATTGCTCACTCTGGGGCATCTGTAGATTTAT1810.14128924484411348No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC1800.1405086412814388No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1760.13738622703074016No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG1730.1350444163427162No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT1680.1311413985293429No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1620.12645777715329493No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1610.12567717359062025No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1590.12411596646527095No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1520.11865174152654832No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT1510.11787113796387368No Hit
TCCCCCTTTATCAAGAAATATTGCTCACTCTGGGGCATCTGTAGATTTAT1500.117090534401199No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1480.11552932727584969No Hit
GTACCGATTTTGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA1480.11552932727584969No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTTGGGAGGAT1450.11318751658782572No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT1390.10850389521177774No Hit
CCCCCCTTTATCAAGAAATATTGCTCACTCTGGGGCATCTGTAGATTTAT1390.10850389521177774No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT1390.10850389521177774No Hit
CTACCGATTGAATGATCCGGTGAGCTATTCGGACTTTCGCTTTGTCGATT1370.10694268808642843No Hit
CTACCGATTGAGCATTAGGGTGAAATCTTCGGACCGTGGCATACTTCTGG1360.10616208452375377No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1330.10382027383572978No Hit
CCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1320.10303967027305513No Hit
CATGGAAGTGGATTGCACCAGAAGTAGATAGTCTAACCTTAGGGAGGGCG1310.10225906671038046No Hit
CCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1300.10147846314770581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)