FastQCFastQC Report
Tue 22 Apr 2025
Site1bb_S139_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1bb_S139_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166905
Total Bases24.9 Mbp
Sequences flagged as poor quality0
Sequence length46-281
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG132737.9524280279200745No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC126287.565980647673827No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA119837.179533267427579No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC85985.151433450166262No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG72844.364159252269255No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG61833.7045025613372875No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG48842.9262155118181No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA44662.675773643689524No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC35602.1329498816692127No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG29421.7626793685030406No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA26421.5829364009466464No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA24411.462508612683862No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG22081.3229082412150623No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC20731.242023905814685No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA18831.1281866930289686No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG18731.1221952607770889No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA18001.0784578053383662No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT16650.9975734699379888No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA15050.9017105539079118No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA14070.8429945178394895No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC13280.795662203049639No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC12750.763907612114676No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA8400.5032803091579042No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC7020.4205985440819628No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC6790.4068182499026392No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT6400.38345166412030796No Hit
GAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGACCTTGA5690.3409124951319613No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA5010.30017075581917857No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA4990.2989724693688026No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG4800.287588748090231No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGACAAGTTCGA4730.2833947455139151No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTACGATTTAT4590.27500674036128336No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG4560.2732093106857194No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC4460.2672178784338396No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4400.26362301908271174No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG4000.2396572900751925No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG3860.23126928492256071No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC3500.2097001288157934No Hit
GAACCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGACCTTGA3390.20310955333872563No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG3360.20131212366316167No Hit
AAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGACCTTGA3320.19891555076240974No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC3170.18992840238459002No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGC3070.18393697013271024No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC3040.18213954045714628No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA3010.18034211078158235No Hit
CTACGGAAACCTTGTTACGACTTCTGCTTCCTCTAAGTGATAAGGCTTGC2830.16955753272819868No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA2830.16955753272819868No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC2810.16835924627782273No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC2780.16656181660225877No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATATGGTTAAC2760.1653635301518828No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2620.15697552499925108No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA2520.15098409274737126No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG2350.14079865791917556No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTAAGGTTCAC2320.13900122824361164No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG2310.13840208501842366No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATGTGGTTTGC2300.13780294179323568No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2290.13720379856804768No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC2110.12641922051466403No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA2100.12582007728947606No Hit
CTACGAAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGATTCAC2010.12042778826278422No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG1980.11863035858722029No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA1980.11863035858722029No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA1930.11563464246128036No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA1790.10724663730864864No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCGC1780.10664749408346066No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA1780.10664749408346066No Hit
CTACGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCACACCGTGGT1760.10544920763308469No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC1760.10544920763308469No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1730.10365177795752074No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAATGATATGGTTTAA1710.10245349150714478No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTTAA1700.1018543482819568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)