FastQCFastQC Report
Tue 22 Apr 2025
Site1bb_S139_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1bb_S139_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166905
Total Bases24.8 Mbp
Sequences flagged as poor quality0
Sequence length49-281
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT135488.11719241484677No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT122567.343099367903898No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT61653.693717983283904No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG61643.6931188400587156No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT50343.016086995596297No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG34502.0670441268985353No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG32951.974176926994398No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT27271.6338635750876247No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT22561.3516671160240856No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG22261.333692819268446No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG21691.2995416554327313No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT20611.2348341871124293No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA19131.146160989784608No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT19081.1431652736586682No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC18811.1269884065785927No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA17091.0239357718462598No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT16981.017345196369192No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA14740.8831371139270843No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC14100.8447919475150535No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA13090.7842784817710674No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT12600.7549204637368563No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT12420.7441358856834726No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA11950.7159761540996376No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT10720.6422815374015158No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG10700.6410832509511398No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG10020.6003415116383571No Hit
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGT8680.5200563194631677No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT8670.5194571762379797No Hit
CTACCGATTGAATGGCTTAGTGAGCTCGAGGGACTGTTGCCGCTTGAGGG7190.43078397891015846No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGT7120.42658997633384266No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG6870.41161139570414307No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG6840.4098139660285791No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG6580.3942362421736916No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT6570.3936370989485036No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA6220.3726670860669243No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG6210.3720679428417363No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT6210.3720679428417363No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT6110.3660765105898565No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5510.33012791707857764No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG5290.31694676612444206No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG5130.30736047452143433No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT5120.30676133129624633No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG5040.3019681854947425No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC4990.2989724693688026No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG4950.29657589646805066No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC4910.29417932356729876No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC4810.28818789131541894No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG4750.2845930319642911No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT4660.2792007429375992No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4600.27560588358647137No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4590.27500674036128336No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4510.2702135945597795No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG4500.26961445133459155No Hit
CTACCGATTGAATGATCCGGTGAGCTATTCGGACTTTCGCTTTGTCGATT4430.26542044875827564No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA4410.2642221623078997No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT4330.2594290165063959No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT4110.24624786555226025No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT3770.22587699589586893No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT3700.22168299331955305No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG3640.21808813396842516No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCTCCTT3560.21329498816692133No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA3560.21329498816692133No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTTAATGTGGTATTCGAGCC3530.21149755849135735No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG3450.2067044126898535No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG3410.2043078397891016No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT3350.20071298043797373No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA3280.19651897786165784No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT3200.19172583206015398No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG3060.18333782690752226No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT3050.18273868368233426No Hit
CCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA2970.1779455378808304No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT2860.17135496240376263No Hit
CTACCGATTGAGTGATCCGGTGAGTAATTCGGACCAGCTGTCGCCTGAGT2830.16955753272819868No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT2820.1689583895030107No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG2750.16476438692669482No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT2680.16057038435037896No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA2670.15997124112519098No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT2580.15457895209849914No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA2510.1503849495221833No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG2460.14738923339624338No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCAGGAATCGCATGAGT2410.14439351727030347No Hit
CTTCCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACCGAGAGCAGCCCCG2400.1437943740451155No Hit
CATGGAAGTGGATTGCACCAGAAGTAGATAGTCTAACCTTAGGGAGGGCG2380.14259608759473952No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG2380.14259608759473952No Hit
CTACCGATTGAACGGTTTAGTGAGTTTCGAGGATCGACGACACTCGAGGT2340.1401995146939876No Hit
CCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA2290.13720379856804768No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT2140.12821665019022796No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT2130.12761750696503998No Hit
CTACCGATTGAGTGCTCAGGTGAATAGTATGGACTGCATCAGTGTTCGGT2110.12641922051466403No Hit
CCCCCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA2040.12222521793834816No Hit
TCCTCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA2040.12222521793834816No Hit
TCCTCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA2010.12042778826278422No Hit
CTACCGATTTCGAGTGATCCGGTGAACCATTCGGACCGGCGCCGCTTCGT1980.11863035858722029No Hit
TCCCCCGTCTCGTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1970.1180312153620323No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1950.11683292891165632No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT1950.11683292891165632No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG1950.11683292891165632No Hit
CCCTCCGTCTCGTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATA1800.10784578053383662No Hit
CCCCCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1790.10724663730864864No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1780.10664749408346066No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT1770.10604835085827267No Hit
CTACCGATTGAGCAATCCGGTGAATATTTCGGACCGGACCCTGTCGGGGC1760.10544920763308469No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1720.10305263473233275No Hit
TCCCCCGTCTCGTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATA1720.10305263473233275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)