FastQCFastQC Report
Tue 22 Apr 2025
Site1b_S84_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1b_S84_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157454
Total Bases29.1 Mbp
Sequences flagged as poor quality0
Sequence length20-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG96356.119247526261638No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA85995.461277579483532No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG84805.385699950461722No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC83795.321554231712119No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC69574.418433320207807No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG43012.7315914489311166No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA33602.133956584145211No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG31722.014556632413276No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG31542.0031247221410697No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA24911.582049360448131No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA21661.3756398694221805No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC17191.0917474309957194No Hit
GAATCAGAAAAGATGTTGAAATAGGACGGGGTCACCTCCTCCTGAAGGAT16771.0650729736939042No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA16491.047290002159361No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT13670.8681900745614592No Hit
AAATCAGAAAAGATGTTGAAATAGGACGGGGTCACCTCCTCCTGAAGGAT12860.81674647833653No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA12650.8034092496856224No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC12170.7729241556264052No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT11770.7475199105770575No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT11260.7151294981391391No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG10420.6617805835355088No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA9750.6192284730778513No Hit
GAACCAGAAAAGATGTTGAAATAGGACGGGGTCACCTCCTCCTGAAGGAT9390.5963646525334383No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT8880.5639742400955199No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC8750.5557178604544819No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT8490.5392051011724059No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA8320.5284082970264331No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT8060.511895537744357No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT7340.46616789665553116No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA7250.46045194151942787No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG7160.45473598638332463No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6740.42806152908150946No Hit
AAACCAGAAAAGATGTTGAAATAGGACGGGGTCACCTCCTCCTGAAGGAT6600.41917004331423785No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC6320.40138707177969435No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC6150.3905902676337216No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6060.38487431249761833No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT6060.38487431249761833No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC5880.37344240222541186No Hit
CTACGAAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGATTCAC5870.3728072960991782No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5750.3651860225843738No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5740.36455091645814014No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5300.3366062469038576No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT5080.3226339121267164No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC4900.31120200185450986No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4600.2921488180674991No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT4580.2908786058150317No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA4380.2781764832903578No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT4340.275636058785423No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG4160.26420414851321655No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4100.26039351175581443No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3910.24832649535737422No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA3880.24642117697867313No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3760.2387999034638688No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG3690.23435416058023295No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC3520.22355735643426017No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT3440.21847650742439062No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3420.21720629517192322No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3310.21022012778335258No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG3230.20513927877348304No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC3200.20323396039478195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2920.18545098886023856No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA2920.18545098886023856No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2900.18418077660777116No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT2670.16957333570439623No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA2670.16957333570439623No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2640.1676680173256951No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA2530.1606818499371245No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGC2390.15179036416985278No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC2390.15179036416985278No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA2340.14861483353868432No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATCAGGTTTAC2320.14734462128621692No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2280.14480419678128215No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT2270.14416909065504846No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC2200.1397233477714126No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2180.13845313551894523No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGC2060.1308318620041409No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTTAA2040.1295616497516735No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG1990.12638611912050504No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA1990.12638611912050504No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC1980.12575101299427133No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGT1920.12194037623686918No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA1890.1200350578581681No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC1880.1193999517319344No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA1840.11685952722699965No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG1840.11685952722699965No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATGTGGTTTGC1720.10923825371219531No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT1700.10796804145972792No Hit
CTACGGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGT1680.10669782920726054No Hit
GAATCAAAACAGGTGTTGGTACAAAATAGGATCTCCTCCTCCCCTTGGGT1670.10606272308102685No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGG1640.10415740470232576No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGC1630.10352229857609208No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTACGATTTAT1630.10352229857609208No Hit
AAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1610.10225208632362469No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC1600.10161698019739097No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG1580.10034676794492359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)