FastQCFastQC Report
Tue 22 Apr 2025
Site1b_S84_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1b_S84_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157454
Total Bases29.1 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT95646.074154991299046No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT84855.3888754810928905No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG67774.304114217485742No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT44152.8039935473217574No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG34492.1904810293800097No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT32402.0577438489971676No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG24201.5369568254855386No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG19631.2467133257967407No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG17821.1317591169484422No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT16481.0466548960331272No Hit
TCCTCCTTTATCAGGAAATCTCGCACACTCAGGCCCATCAGTAGATATAG15200.9653613118752143No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGAACTTTGTTGGCT14710.9342411116897634No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA14280.9069315482617145No Hit
TCCCCCTTTATCAGGAAATCTCGCACACTCAGGCCCATCAGTAGATATAG13210.8389751927547093No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT12720.8078549925692582No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA11870.7538709718393943No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG10480.665591220292911No Hit
CCCTCCTTTATCAGGAAATCTCGCACACTCAGGCCCATCAGTAGATATAG10380.6592401590305741No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG10330.6560646283994056No Hit
CCCCCCTTTATCAGGAAATCTCGCACACTCAGGCCCATCAGTAGATATAG10220.6490784610108349No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC10040.6376465507386284No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA9630.611607199563047No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG9140.580486999377596No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG9130.5798518932513622No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT9070.5760412564939601No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG8800.5588933910856504No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA8740.5550827543282483No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8610.5468263746872103No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC8540.5423806318035743No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG8380.5322189337838352No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG7630.48458597431630823No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG7490.47569448854903656No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG7260.4610870476456616No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA7260.4610870476456616No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6990.44393918223735185No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG6890.4375881209750149No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT6880.43695301484878124No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6750.4286966352077432No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT6670.42361578619787366No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG6630.4210753616929389No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT6400.4064679207895639No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG6380.4051977085370966No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6360.4039274962846291No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG6260.39757643502229223No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG6100.38741473700255313No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG5960.3785232512352814No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5880.37344240222541186No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA5320.337876459156325No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5300.3366062469038576No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT5300.3366062469038576No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG5300.3366062469038576No Hit
CTACCGATTGAGTGCTCAGGTGAATAGTATGGACTGCATCAGTGTTCGGT5210.3308902917677544No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT5140.32644454888411856No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA5070.32199880600048264No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG4900.31120200185450986No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4860.3086615773495751No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC4810.3054860467184067No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC4750.3016754099610045No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG4660.29595945482490127No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4640.29468924257243384No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT4580.2908786058150317No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4440.28198712004775994No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4280.2718254220280209No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG4230.2686498913968524No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT4210.267379679144385No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT4170.26483925463945024No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG3880.24642117697867313No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA3700.23498926670646667No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG3500.22228714418179277No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG3460.219746719676858No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG3420.21720629517192322No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG3320.2108552339095863No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG3100.19688289913244503No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT3100.19688289913244503No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT2970.18862651949140702No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT2880.18291056435530376No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG2760.17528929084049946No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT2720.1727488663355647No Hit
CCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2700.1714786540830973No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC2610.16576269894699402No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT2580.16385738056829297No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT2570.16322227444205928No Hit
CCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2400.15242547029608647No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG2300.14607440903374955No Hit
CTACCGATTGAGTGATCTGGTGAACCTTCTGGACTGCGACCGTCCTCGAG2260.14353398452881477No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2260.14353398452881477No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT2000.12702122524673873No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC1990.12638611912050504No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT1990.12638611912050504No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC1940.12321058848933657No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG1930.12257548236310287No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTTAATGTGGTATTCGAGCC1910.1213052701106355No Hit
CTACCGATTGAACGGCTTAGTGAGTTTCGAGGATTTGGGTACTGTTCGGT1890.1200350578581681No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1880.1193999517319344No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT1860.11812973947946702No Hit
CATGGGAGTGGGTTGCAAAAGAAGTGGGTAGCATAACCTTCGGGAGTGCG1830.11622442110076595No Hit
CTACCGATTGAACGTGCTGGTGAAGAGTTTGGATTGGAGTTTTGGCTAGG1830.11622442110076595No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATGGATGGATTGTG1810.11495420884829854No Hit
CTTCCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACCGAGAGCAGCCCCG1800.11431910272206484No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1800.11431910272206484No Hit
TCCTCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG1770.11241378434336377No Hit
CTACCGATTGAATGATCCGGTGAGCTATTCGGACTTTCGCTTTGTCGATT1760.11177867821713008No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT1730.109873359838429No Hit
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG1710.10860314758596161No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG1660.10542761695479315No Hit
TCCCCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG1650.10479251082855946No Hit
CCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1640.10415740470232576No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCAGGAATCGCATGAGT1580.10034676794492359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)