FastQCFastQC Report
Tue 22 Apr 2025
Site1a_S83_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1a_S83_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220798
Total Bases34.5 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT143706.508211125100772No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT143426.495529850813867No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT95164.309821646935208No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT83433.7785668348445185No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG44492.0149639036585474No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT44342.008170363861992No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG44202.0018297267185394No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG42681.9329885234467703No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG36631.6589824183190067No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT35851.6236560113769147No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG31611.4316252864609282No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT29071.3165880125725777No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA28391.2857906321615231No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT26411.1961159068469822No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT25491.154448862761438No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT25331.147202420311778No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT21470.9723819962137339No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA21050.9533600847833766No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG20740.9393201025371606No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG20540.9302620494750857No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT17820.807072527830868No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC15510.7024520149639036No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG15140.6856946167990652No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG15000.6793539796556127No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA14780.6693901212873306No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA13870.62817597985489No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT13640.6177592188335039No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT13430.6082482631183254No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG12690.5747334667886485No Hit
CTACCGATTGAGTGATCCGGTGAGTAATTCGGACTGCAGCAGTGTTCAGT12200.5525412367865651No Hit
CTACCGATTGGATGATCCGGTGAGCTATTCGGACTTTCGCTTAGTCGATT11440.5181206351506807No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG11440.5181206351506807No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT11110.5031748475982571No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC10430.47237746718720275No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC10240.46377231677823166No Hit
CTACCGATTGAACGGCTTAGTGAGTTTCGAGGATTTGGGTACTGTTCGGT9750.44158008677614835No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC9530.431616228407866No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACCTCCTTCGTACCAGTT9520.43116332575476224No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG9470.42889881248924355No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA9140.4139530249368201No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT8940.40489497187474527No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT8630.3908549896285292No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCTCCTT7960.3605105118705786No Hit
CTACCGATTGAATGGCTTAGTGAGCTTGCTGGAGTGATGCACTTGTCGGG7730.3500937508491925No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG7280.3297131314595241No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT7010.31748475982572305No Hit
CTACCGATTTAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT6730.3048034855388183No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG6700.30344477757950705No Hit
CTACCGATTGAGCAATCCGGTGAATATTTCGGACCGGACCCTGTCGGGGC6680.30253897227329957No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC6660.3016331669670921No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA6220.28170545023052745No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG5960.2699299812498302No Hit
CTACCGATTGAACATTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT5940.2690241759436227No Hit
CTACCGATTGTGTGTTTTGGTGAACATTTGTGAGCTACGATGCATAGAGG5770.26132483084085906No Hit
CAACCGAGTTATGTAGGGATGAAGCTCACTAGAGAAAAATCTTTTCATGA5700.2581545122691329No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA5690.2577016096160291No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC5390.2441145300229169No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT5320.2409442114511907No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG5310.24049130879808694No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT5250.2377738928794645No Hit
CTACCGATTGAATGGCTTAGTGAGATCTCCGGATTGGCTTTGGGAAGCTG5220.23641518492015326No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5060.2291687424704934No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGATCCCTGCATGGCG5050.22871583981738966No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGTGGCTTGTGCGCTT4890.22146939736772978No Hit
CTACCGATTGAATGGTTTGATGAATTGCGCGGACTATCCGTCTTGGGGGT4860.22011068940841855No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4810.21784617614289983No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG4730.2142229549180699No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG4570.20697651246841006No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4570.20697651246841006No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4400.19927716736564643No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4370.1979184594063352No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT4310.19520104348771275No Hit
GTACCGATTTCGAGTTGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA4290.19429523818150526No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG4250.1924836275690903No Hit
CTACCGATTGAATGGCTTAGGGAGATTTCCGGATTGGCGTTAGGAAGCCG4010.1816139638946005No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3850.1743675214449406No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT3820.1730088134856294No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG3760.17029139756700695No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3680.166668176342177No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG3450.15625141532079095No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG3360.15217529144285727No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG3350.15172238878975353No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG3150.1426643357276787No Hit
CTACCGATTGAATGGCTTAGTGAGCTCGAGGGACTGTTGCCGCTTGAGGG3130.1417585304214712No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3080.1394940171559525No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3040.13768240654353753No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3020.13677660123733004No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG2990.13541789327801884No Hit
CTACCGATTGAGTGATCTGGTGAACCTTCTGGACTGCGACCGTCCTCGAG2920.13224757470629264No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT2910.1317946720531889No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2870.12998306144077393No Hit
CATGGAAGTGGATTGCACCAGAAGTAGATAGTCTAACCTTAGGGAGGGCG2870.12998306144077393No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG2830.12817145082835896No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT2790.12635984021594399No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTT2730.12364242429732154No Hit
CTACCGATTGAACGGTTTAGTGAGTTTCGAGGATCGACGACACTCGAGGT2670.12092500837869909No Hit
CTACCGATTGAATGATCCGGTGAGCTATTCGGACTTTCGCTTTGTCGATT2660.12047210572559534No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT2640.11956630041938786No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG2620.11866049511318037No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATGGATGGATTGTG2590.11730178715386914No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG2590.11730178715386914No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTCCGAAGGTT2560.11594307919455792No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG2560.11594307919455792No Hit
CTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGGCGTTGAGAAGCCG2430.11005534470420927No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC2390.1082437340917943No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2380.10779083143869056No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT2350.10643212347937933No Hit
CTACCGATTGAGTGGTTCGGTGAACTTTTTGGACTGCGCAGGCTTTAGTC2350.10643212347937933No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT2340.10597922082627559No Hit
CTACCGATTGAACGTGCTGGTGAAGAGTTTGGATTGGAGTTTTGGCTAGG2250.10190309694834193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)