FastQCFastQC Report
Tue 22 Apr 2025
Site1U_S114_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1U_S114_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42474
Total Bases9.3 Mbp
Sequences flagged as poor quality0
Sequence length50-281
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG21094.96539059189151No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG17234.0565993313556525No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG16333.8447049959975512No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG15393.623393134623534No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG9052.130715261100909No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7351.730470405424495No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6951.6362951452653387No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6371.4997410180345623No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4721.111268069878043No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3440.8099072373687433No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2620.6168479540424731No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2550.6003672835146208No Hit
TCCCCCCTTATCTTCAAATATTGCACAAGAAGGTGCTAGAATGGATTTAG2120.4991288788435278No Hit
TCCTCCCTTATCTTCAAATATTGCACAAGAAGGTGCTAGAATGGATTTAG1690.3978904741724349No Hit
CCCCCCCTTATCTTCAAATATTGCACAAGAAGGTGCTAGAATGGATTTAG1640.3861185666525404No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1590.37434665913264586No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1520.3578659886047935No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1420.3343221735650045No Hit
CCCTCCCTTATCTTCAAATATTGCACAAGAAGGTGCTAGAATGGATTTAG1330.31313274002919433No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1320.3107783585252154No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1260.296652069501342No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1230.2895889249894053No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1190.2801713989734897No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1120.26369072844563735No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG1110.2613363469416584No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1100.2589819654376795No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT1060.2495644394217639No Hit
TCCTCCACTTAGAGGCAATTTAGCACATGCAGGAGCATCAGTAGATTTTG1050.247210057917785No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG990.23308376889391155No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG960.22602062438197484No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG960.22602062438197484No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG940.22131186137401704No Hit
CCCTCCACTTAGAGGCAATTTAGCACATGCAGGAGCATCAGTAGATTTTG850.20012242783820688No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT850.20012242783820688No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG850.20012242783820688No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG840.19776804633422798No Hit
CCCCCCTTATCTTCAAATATTGCACAAGAAGGTGCTAGAATGGATTTAGC820.1930592833262702No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG820.1930592833262702No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG800.18835052031831237No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG780.18364175731035456No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG780.18364175731035456No Hit
CCCCCCACTTAGAGGCAATTTAGCACATGCAGGAGCATCAGTAGATTTTG770.18128737580637566No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG750.17657861279841786No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG720.16951546828648115No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG710.16716108678250224No Hit
TCCTCCTTTATCTGCGTTGCCTCAGGCCATGCCAGGTTCTGGAACGGGTA660.1553891792626077No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG660.1553891792626077No Hit
TCCCCCACTTAGAGGCAATTTAGCACATGCAGGAGCATCAGTAGATTTTG650.1530347977586288No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG640.1506804162546499No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG630.148326034750671No Hit
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACATACA620.1459716532466921No Hit
TCCTCCATTATCTAGTATAACAGCACACTCAGGAGGTGCTGTAGATTTAG610.1436172717427132No Hit
TCCCCCTTTGTCAAGTGGCAATTCTCATTCAGGGCCTGCTGTAGATTTAG600.14126289023873428No Hit
CCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG590.13890850873475538No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG540.12713660121486087No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG540.12713660121486087No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACATACA530.12478221971088195No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG490.11536469369496634No Hit
TCCCCCATTATCTAGTATAACAGCACACTCAGGAGGTGCTGTAGATTTAG490.11536469369496634No Hit
TCCTCCTTTGTCAAGTGGCAATTCTCATTCAGGGCCTGCTGTAGATTTAG490.11536469369496634No Hit
CCCTCCATTATCTAGTATAACAGCACACTCAGGAGGTGCTGTAGATTTAG490.11536469369496634No Hit
CCCCCCATTATCTAGTATAACAGCACACTCAGGAGGTGCTGTAGATTTAG460.10830154918302962No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT430.10123840467109291No Hit
CCCCCCTTTGTCAAGTGGCAATTCTCATTCAGGGCCTGCTGTAGATTTAG430.10123840467109291No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)