FastQCFastQC Report
Tue 22 Apr 2025
Site1L_S101_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1L_S101_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49765
Total Bases8.9 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT558711.226765799256505No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG45289.098764191700996No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT21424.304229880438059No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT13632.7388727016979804No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT12662.543956596001206No Hit
CTACCGATTGGCTGTGGGATAGAGTTTGGGGGAGGGTGTCTGTTCTTGGC8721.7522355068823472No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT8471.7019993971666834No Hit
CTACCGATTGGGTGTACCAGTGAGATCCTTGGACTGGCGAGCCTTGTGCG8071.6216216216216217No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT6731.3523560735456646No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG5971.1996383000100472No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT5701.1453833015171306No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5221.0489299708630564No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5051.0147694162564052No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG4981.0007033055360193No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG4970.9986938611473928No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT4930.9906560835928866No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG4870.9785994172611273No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT4800.9645333065407414No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT4210.8459760876117753No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG4000.8037777554506178No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT3920.7877022003416054No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT3700.7434944237918215No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT3620.7274188686828091No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG3500.7033055360192907No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3250.6530694263036271No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3190.6410127599718678No Hit
CTACCGATTGAATGGTTTAGTGAGATCTCCGGATTGGCACCATCGCGGCT3040.6108710941424695No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2470.49633276399075654No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2340.4702099869386115No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2170.43604943233196025No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT1950.3918416557821762No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1870.37576610067316385No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1630.32753943534612684No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1600.3215111021802472No Hit
CTACCGATTGAATGGTTTAGTGAGGTTATCGGATCCTAAGACCAGACATG1600.3215111021802472No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1590.31950165779162065No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1540.30945443584848786No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG1540.30945443584848786No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA1510.3034261026826082No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC1450.291369436350849No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1450.291369436350849No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1440.28935999196222245No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1430.2873505475735959No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT1410.2833316587963428No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC1380.27730332563046317No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1350.27127499246458353No Hit
CATGGGAATTGAACTCATTCGAAGCGGGAATGCTAAAGTAGCTACCCTCC1330.2672561036873305No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT1320.26524665929870395No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1310.2632372149100774No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG1300.2612277705214508No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT1290.25921832613282425No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGCGACGCCAGAGCGC1280.2572088817441977No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGTATGCTGAG1270.25519943735557116No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACGTCGAGAGGTT1260.2531899929669446No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1260.2531899929669446No Hit
CCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1190.2391238822465588No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1170.23510499346930575No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT1150.23108610469205265No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1140.2290766603034261No Hit
CCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1070.2150105495830403No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1060.21300110519441376No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1010.202953883251281No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1000.20094443886265445No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT990.1989349944740279No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT950.19089721691952177No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCGGTGCCTTA950.19089721691952177No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA930.18687832814226865No Hit
CTACCGATTGGCTGTGGGATAGAGCTTGGGAGAGGGAAGCTCGTTCTTGG920.1848688837536421No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT900.180849994976389No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT900.180849994976389No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT880.17683110619913595No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGGCCTGTCTTGGTT850.1708027730332563No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT850.1708027730332563No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG800.1607555510901236No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT760.15271777353561738No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG740.14869888475836432No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG710.14267055159248468No Hit
CTACCGATTGAATGGTTTAGTGAGTTCCTCGGATTGGTCCCGGCATGGTG700.14066110720385813No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG690.13865166281523159No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG670.13463277403797852No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTGCTTACGCTT670.13463277403797852No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGTGGCTTGTGCGCTT660.13262332964935197No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG660.13262332964935197No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG660.13262332964935197No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT660.13262332964935197No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGACCGGCCCCTTCACGGCG650.1306138852607254No Hit
CTACCGATTGAACGTTCCGGTGAGACCTTTGGATCTGCTGATGTGGGGCG640.12860444087209885No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG630.1265949964834723No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGAATTAGGTTAGTT630.1265949964834723No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG600.12056666331759268No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG580.1165477745403396No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT580.1165477745403396No Hit
TCCCCCTCTCTCAAGTAATGTTGCACATGCGGGAAGATCGGTTGACTTTG570.11453833015171305No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATAGATGGACTGTG570.11453833015171305No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG540.10850999698583343No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT530.10650055259720688No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT530.10650055259720688No Hit
CCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG530.10650055259720688No Hit
CTACCGATTGAATGGTTTAGTGAGGTCATCGGATTGGGTGCATGAGAGTG520.10449110820858033No Hit
TCCCCCTTTATCATCTAACATTGCACACGGAGGAGGCTCCGTAGACTTAG520.10449110820858033No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA520.10449110820858033No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATGGATGGATTGTG520.10449110820858033No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG510.1024816638199538No Hit
TCCTCCTCTCTCAAGTAATGTTGCACATGCGGGAAGATCGGTTGACTTTG500.10047221943132723No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCAGTTCACTT500.10047221943132723No Hit
CCCCCCTCTCTCAAGTAATGTTGCACATGCGGGAAGATCGGTTGACTTTG500.10047221943132723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)