FastQCFastQC Report
Tue 22 Apr 2025
Site1J_S99_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1J_S99_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8551
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length55-281
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT3343.90597590925038No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT2322.7131329669044555No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA2192.5611039644486024No Hit
AAATCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT1992.327213191439598No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1561.8243480294702372No Hit
GAACCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT1331.555373640509882No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1291.5085954859080808No Hit
GAATCAGAACAAGTGTTGATAAAGAATCACATCTCCCCCTCCAGCAGGAT1121.3097883288504268No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1111.2980937901999767No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1051.2279265582972752No Hit
AAACCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT921.0758975558414219No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT921.0758975558414219No Hit
AAATCAGAACAAGTGTTGATAAAGAATCACATCTCCCCCTCCAGCAGGAT881.029119401239621No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT871.0174248625891709No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT790.9238685533855688No Hit
GAACCAGAACAAGTGTTGATAAAGAATCACATCTCCCCCTCCAGCAGGAT770.9004794760846684No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT740.8653958601333177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG700.8186177055315168No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT670.783534089580166No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT650.7601450122792656No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT560.6548941644252134No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT560.6548941644252134No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT560.6548941644252134No Hit
GAATCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT540.631505087124313No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT540.631505087124313No Hit
GAATCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT510.5964214711729622No Hit
GAATCAAAATAGATGTTGGTAGAGTAAGGGGTCCCCTCCTCCCCTAGGGT490.5730323938720617No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT460.5379487779207109No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT460.5379487779207109No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT460.5379487779207109No Hit
AAATCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT440.5145597006198105No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT410.47947608466845987No Hit
GAACCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT400.46778154601800953No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT400.46778154601800953No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG390.4560870073675593No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC380.4443924687171091No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA380.4443924687171091No Hit
AAATCAAAATAGATGTTGGTAGAGTAAGGGGTCCCCTCCTCCCCTAGGGT360.4210033914162086No Hit
AAACCAGAACAAGTGTTGATAAAGAATCACATCTCCCCCTCCAGCAGGAT360.4210033914162086No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT360.4210033914162086No Hit
GAACCAAAATAGATGTTGGTAGAGTAAGGGGTCCCCTCCTCCCCTAGGGT340.3976143141153081No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT340.3976143141153081No Hit
AAATCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT330.38591977546485795No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT320.37422523681440767No Hit
GAACCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT320.37422523681440767No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG310.36253069816395744No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT310.36253069816395744No Hit
GAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT310.36253069816395744No Hit
AAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT300.3508361595135072No Hit
GAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT290.33914162086305694No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT280.3274470822126067No Hit
AAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT260.3040580049117062No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA240.28066892761080575No Hit
AAACCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT240.28066892761080575No Hit
GAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT240.28066892761080575No Hit
AAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT230.26897438896035547No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG220.25727985030990524No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC220.25727985030990524No Hit
GAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT220.25727985030990524No Hit
AAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT210.24558531165945505No Hit
AAACCAAAATAGATGTTGGTAGAGTAAGGGGTCCCCTCCTCCCCTAGGGT200.23389077300900477No Hit
GAACCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT200.23389077300900477No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT200.23389077300900477No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT200.23389077300900477No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT190.22219623435855454No Hit
GAATCAAAACAAATGCTGATAAAGAACAGGATCTCCACCACCAGCAGGAT180.2105016957081043No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT180.2105016957081043No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT180.2105016957081043No Hit
AAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT170.19880715705765406No Hit
GAATCAAAATAAATGTTGATACAATACAGGGTCTCCTCCTCCTGCCGGAT170.19880715705765406No Hit
GAATCAGAAAAGATGCTGGTAAAGAACAGGATCACCACCGAAGATTGGAT170.19880715705765406No Hit
AAACCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT160.18711261840720383No Hit
AAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT160.18711261840720383No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT160.18711261840720383No Hit
AAATCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT150.1754180797567536No Hit
GAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT150.1754180797567536No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA150.1754180797567536No Hit
GAATCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT150.1754180797567536No Hit
GAATCAGAATAAATGCTGGTATAAAATAGGATCACCACCACCTGCAGGAT150.1754180797567536No Hit
GAACCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT140.16372354110630336No Hit
GAATCAGAAAAGGTGTTGGTATAAAACTGGATCACCACCTCCTGAAGGAT140.16372354110630336No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT140.16372354110630336No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT140.16372354110630336No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG140.16372354110630336No Hit
AAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT130.1520290024558531No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT130.1520290024558531No Hit
AAACCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT120.14033446380540288No Hit
GAATCAGAACAAATGTTGGTACAATACAGGATCCCCTCCACCTGAAGGAT120.14033446380540288No Hit
GAACCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT120.14033446380540288No Hit
GAACCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT120.14033446380540288No Hit
GAATCAGAAAAGATGCTGATACAAGATAGGGTCTCCACCACCTGCGGGGT120.14033446380540288No Hit
AAATCAAAATAGATGTTGGTAGAGGATGGGATCCCCCCCTCCAGCTGGAT120.14033446380540288No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT110.12863992515495262No Hit
GAATCAAAATAAATGTTGATATAAAATTGGGTCTCCTCCGCCTGCTGCAT110.12863992515495262No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT110.12863992515495262No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT110.12863992515495262No Hit
GAACCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT110.12863992515495262No Hit
AAACCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT110.12863992515495262No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG110.12863992515495262No Hit
GAATCAAAATAAATGTTGGAAAAGAATAGGATCTCCACCCCCAGCTGGAT110.12863992515495262No Hit
GAATCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT110.12863992515495262No Hit
AAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT110.12863992515495262No Hit
AAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT110.12863992515495262No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA100.11694538650450238No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA100.11694538650450238No Hit
AAATCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT100.11694538650450238No Hit
GAACCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT100.11694538650450238No Hit
GAATCAAAATAAATGTTGATAAAGTATAGGATCACCCCCACCTTCGTAAT100.11694538650450238No Hit
AAACCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT90.10525084785405216No Hit
GAACCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT90.10525084785405216No Hit
GAACCAGAAAAGGTGTTGGTATAAAACTGGATCACCACCTCCTGAAGGAT90.10525084785405216No Hit
AAATCAGAAAAGGTGTTGGTATAAAACTGGATCACCACCTCCTGAAGGAT90.10525084785405216No Hit
GAATCAAAATAAATGCTGGTATAAAATAGGATCCCCTCCTCCTCTTCTAT90.10525084785405216No Hit
AAACCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT90.10525084785405216No Hit
AAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT90.10525084785405216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)