FastQCFastQC Report
Tue 22 Apr 2025
Site1J_S99_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1J_S99_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8551
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length55-281
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCTTTATCTGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG1962.292129575488247No Hit
TCCCCCTTTATCTGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG1451.6957081043152848No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1401.6372354110630336No Hit
CCCTCCTTTATCTGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG1311.5319845632089815No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1201.4033446380540289No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1131.3214828675008772No Hit
CCCCCCTTTATCTGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG1121.3097883288504268No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCACAATCGCGGCC1051.2279265582972752No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG961.122675710443223No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG780.9121740147351186No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG780.9121740147351186No Hit
TCCTCCTTTAAGTAGTATATTAGCTCATTCAACACCAGGTGTAGATTTGG750.8770903987837679No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG710.830312244181967No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG700.8186177055315168No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG650.7601450122792656No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG610.7133668576774647No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG610.7133668576774647No Hit
TCCCCCTTTAAGTAGTATATTAGCTCATTCAACACCAGGTGTAGATTTGG610.7133668576774647No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG590.6899777803765641No Hit
CCCTCCTTTAAGTAGTATATTAGCTCATTCAACACCAGGTGTAGATTTGG580.6782832417261139No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG550.6431996257747632No Hit
CCCCCCTTTAAGTAGTATATTAGCTCATTCAACACCAGGTGTAGATTTGG550.6431996257747632No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG520.6081160098234124No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG520.6081160098234124No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG480.5613378552216115No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.5613378552216115No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG410.47947608466845987No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG410.47947608466845987No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG400.46778154601800953No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG400.46778154601800953No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG400.46778154601800953No Hit
TCCTCCGTTAAGAAGAAACATTGCCCATGGGGGTGCATCTGTAGATCTCG360.4210033914162086No Hit
TCCCCCGTTAAGAAGAAACATTGCCCATGGGGGTGCATCTGTAGATCTCG340.3976143141153081No Hit
CCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG330.38591977546485795No Hit
CTACCGATTGAATGGTTTAGTGAGATGTTCGGATTGGCGCCACTGCTCTT330.38591977546485795No Hit
CCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG310.36253069816395744No Hit
TCCTCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG310.36253069816395744No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG310.36253069816395744No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG310.36253069816395744No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG290.33914162086305694No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT290.33914162086305694No Hit
CCCTCCGTTAAGAAGAAACATTGCCCATGGGGGTGCATCTGTAGATCTCG280.3274470822126067No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG270.3157525435621565No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT270.3157525435621565No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT260.3040580049117062No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG260.3040580049117062No Hit
TCCCCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG250.292363466261256No Hit
CCCCCCGTTAAGAAGAAACATTGCCCATGGGGGTGCATCTGTAGATCTCG250.292363466261256No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG250.292363466261256No Hit
CCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG240.28066892761080575No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG240.28066892761080575No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT240.28066892761080575No Hit
CCCTCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG230.26897438896035547No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG220.25727985030990524No Hit
CCCCCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG210.24558531165945505No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG210.24558531165945505No Hit
TCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG210.24558531165945505No Hit
TCCTCCCCTTAGCCAAAACTTAGCGCATAGCGGGAGGTCTGTAGATTTTG200.23389077300900477No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG200.23389077300900477No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG200.23389077300900477No Hit
TCCTCCATTATCAAGTGCAAACGCTCATTCAGGACCTTCAGTAGATTTAG190.22219623435855454No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG190.22219623435855454No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG190.22219623435855454No Hit
TCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG190.22219623435855454No Hit
TCCCCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG180.2105016957081043No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG170.19880715705765406No Hit
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG160.18711261840720383No Hit
CCCCCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG160.18711261840720383No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG150.1754180797567536No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG150.1754180797567536No Hit
CCCTCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG150.1754180797567536No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG140.16372354110630336No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG140.16372354110630336No Hit
TCCCCCATTATCAAGTGCAAACGCTCATTCAGGACCTTCAGTAGATTTAG140.16372354110630336No Hit
TCCCCCATTAAGTAGCATTAGTGCTCACCCAGGTGGTGCAGTAGATTTAG130.1520290024558531No Hit
TCCTCCATTAAGTAGCATTAGTGCTCACCCAGGTGGTGCAGTAGATTTAG130.1520290024558531No Hit
TCCTCCTTTAAGTAGTACGATGGCTCATCCTGGTGGTGCCGTAGATTTAG130.1520290024558531No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG130.1520290024558531No Hit
CCCTCCTTTAGCGAGTGTACAAGCACACTCAGGACCATCTATTGATTTAG130.1520290024558531No Hit
TCCTCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG130.1520290024558531No Hit
CCCTCCCCTTAGCCAAAACTTAGCGCATAGCGGGAGGTCTGTAGATTTTG130.1520290024558531No Hit
TCCTCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG120.14033446380540288No Hit
CCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG120.14033446380540288No Hit
CCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG120.14033446380540288No Hit
TCCTCCTCTAAGCTCAATTCAAAGTCACTCAGGTGGTGCTGTAGATTTAG120.14033446380540288No Hit
CCCCCCTTTAAGTAGTACGATGGCTCATCCTGGTGGTGCCGTAGATTTAG120.14033446380540288No Hit
TCCTCCTTTAAGTAGTGCACAGGCTCATTCTGGGCCTGCCGTAGACCTTG120.14033446380540288No Hit
TCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG110.12863992515495262No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG110.12863992515495262No Hit
TCCCCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG110.12863992515495262No Hit
CCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG110.12863992515495262No Hit
TCCTCCGTTATCGGGGCCTATAGCACATTCAGGTGCTTCAGTAGATTTGG110.12863992515495262No Hit
CCCTCCTTTAAGTAGTGCACAGGCTCATTCTGGGCCTGCCGTAGACCTTG110.12863992515495262No Hit
TCCTCCCTTAGCCAAAACTTAGCGCATAGCGGGAGGTCTGTAGATTTTGC110.12863992515495262No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT110.12863992515495262No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG110.12863992515495262No Hit
CCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG110.12863992515495262No Hit
TCCCCCTTTATCTGGACCACAACTGCACTCAGGAGGTTCTGTAGATATGG110.12863992515495262No Hit
TCCCCCCCTTAGCCAAAACTTAGCGCATAGCGGGAGGTCTGTAGATTTTG110.12863992515495262No Hit
TCCTCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG110.12863992515495262No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG100.11694538650450238No Hit
TCCTCCATTAAGTAGTATTGCTTCTCACTCAGGTGGAAGTGTAGACCTAG100.11694538650450238No Hit
TCCCCCTTAGCCAAAACTTAGCGCATAGCGGGAGGTCTGTAGATTTTGCC100.11694538650450238No Hit
CCCCCCTTTATCTGGACCACAACTGCACTCAGGAGGTTCTGTAGATATGG100.11694538650450238No Hit
TCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG100.11694538650450238No Hit
CCCTCCATTATCAAGTGCAAACGCTCATTCAGGACCTTCAGTAGATTTAG100.11694538650450238No Hit
TCCCCCTTTATCTTCTAATATTGCACACGGAGGAGGCTCCGTAGACTTAG100.11694538650450238No Hit
TCCTCCATTAGCAAGTATTGCTTTTCACTCAGGAGGAGCAGTTGACTGTG90.10525084785405216No Hit
CCCTCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG90.10525084785405216No Hit
CCCCCCTTAGCCAAAACTTAGCGCATAGCGGGAGGTCTGTAGATTTTGCC90.10525084785405216No Hit
TCCCCCATTAAGTAGTATTGCTTCTCACTCAGGTGGAAGTGTAGACCTAG90.10525084785405216No Hit
CCCTCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG90.10525084785405216No Hit
TCCTCCTCTAAGAAGAAATTTAGCCCATAGAGGGAAATCTGTAGATTTTG90.10525084785405216No Hit
CCCTCCTCTATCTACTTCTTTCCTATCTCTATCCCCATCTTCTATGTACT90.10525084785405216No Hit
CCCCCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG90.10525084785405216No Hit
TCCCCCTTTAAGTAGTACGATGGCTCATCCTGGTGGTGCCGTAGATTTAG90.10525084785405216No Hit
TCCCCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG90.10525084785405216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)