FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5z_S131_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5z_S131_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518860
Total Bases156.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC10909021.024939289981884No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC7359314.18359480399337No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC313636.0445977720387No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC256654.946420999884362No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC173003.3342327410091355No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC71351.3751300928959642No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC34830.671279343175423No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC20080.3870022742165517No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17070.32899047912731755No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC16340.31492117334155645No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC15160.2921790078248468No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC12850.24765832787264386No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA12080.2328181012219096No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10300.1985121227306017No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC9970.19215202559457273No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC9850.1898392629996531No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC9190.1771190687275951No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC8860.1707589715915661No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC8170.15746058667077822No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC6890.1327911189916355No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC6680.12874378445052614No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC6150.11852908298963111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)