FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5w_S128_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5w_S128_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Total Bases3.9 kbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC323.076923076923077No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC323.076923076923077No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAAAGGTGCTGGTAAAGCACAGG17.6923076923076925No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAATAGGTGTTGGTATAGAACTGG17.6923076923076925No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAACAAATGTTGATATAATACAGG17.6923076923076925No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC17.6923076923076925No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAATAAATGCTGATATAAAATCGG17.6923076923076925No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC17.6923076923076925No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGATACAATACAGG17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)