FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5v_S127_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5v_S127_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83172
Total Bases25 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1797821.615447506372337No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1625819.54744385129611No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC69848.397056701774636No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC38194.591689510893089No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC34994.2069446448323955No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC15491.8624056172750445No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC13631.6387726638772664No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC9731.1698648583657962No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC7930.9534458712066561No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC7430.8933294858846728No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC7080.8512480161592845No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC6570.7899293031308614No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC3210.38594719376713316No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA2700.32462848073871015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2650.3186168422065118No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC2500.30058192660991684No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC2370.2849516664262011No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC2020.24287019670081278No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTAT1860.22363295339777808No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC1500.18034915596595008No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1440.17313518972731207No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTTCTTC1420.17073053431443275No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC1400.1683258789015534No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC1290.15510027413071706No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTCACGACTTCACCTTC1270.15269561871783774No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC1200.14427932477276006No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG1140.13706535853412208No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1080.12985139229548406No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG890.10700716587313037No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG880.1058048381666907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)