FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5v_S127_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5v_S127_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83172
Total Bases25 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGCCTCG980311.786418506228058No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTCG62867.557831962679748No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTCCTC39734.776847977684798No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTATTT32713.932813927764151No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGATCCTC27593.317222142067042No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGATTCGGTGAAAATCTC25023.008223921512047No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGTTTTAGTGAGGTCCTC19222.3108738517770404No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTC18522.2267109123262636No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTGTTT11951.4367816091954022No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACATTTTAGTGAGGTCCTC11321.3610349636897032No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGGATGGTCCGGTGAACTGGTC10801.2985139229548406No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCCTTTG9311.1193670946953302No Hit
GTACACACCGCCCGTCGCACCAACCGATTGAATGGTTTGGTGAAATATTG9081.0917135574472179No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCTCTCT8631.0376088106574328No Hit
GTACACACCGCCCGTCGCAGCTACTGATTGAATACATCGGTGAAATATTG8130.9774924253354494No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTCGAGTGGTCCGGTGAATCTT7650.9197806954263454No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGATTTCC7630.9173760400134661No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGCGTTC7610.9149713846005868No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGTGTTGGTGAGGAGCTC7350.8837108642331554No Hit
GTACACACCGCCCGTCAAACCATCTTAGTTGGGGGTGGGTGAGGCTGCGC5450.6552686000096186No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAATCTTC4940.5939498869811956No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGACTCG4870.585533593036118No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTGAGTGGTCCGGTGAATCTTT4510.54224979560429No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAGCATTAGGGTGAAATCTTC4440.5338335016592122No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGGATGGTCCGGTGAAATGTTC3790.45568220074063387No Hit
GTACACACCGCCCGTCAAACCATCTTAGTTGTGGGTGGGTGAGGCTACCT3770.4532775453277545No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTTG3680.4424565959697976No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATCCTC3620.4352426297311595No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTCGAGTGGTCCGTTGAATCTT3250.39075650459289185No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGAATTAGTGAGGCCTTT3200.38474486606069347No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTCGAGTGGTCCGGTGAGCCTT2940.35348434569326215No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGTTTCGGTGAAGATTCC2890.3474727071610638No Hit
GTACACACCGCCCGTCGCTACTACCGATCGAGTGGATTAGTGAGGACCTC2800.3366517578031068No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGATTCGGTGAATTATCT2620.3150098590871928No Hit
GTACACACCGCCCGTCGCATCTACCGATTGAATGGCCCGGTGAAGACCTG2550.30659356514211517No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGATCCTC2520.3029865820227961No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATTTACTAGTGAGACAATA2480.29817727119703746No Hit
GTACACACCGCCCGTCGCTACTACCGACTGGTAGATATGGTGAATGGATT2310.2777377001875631No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAGTGGATTAGTGAGGACATC2220.2669167508296061No Hit
GTACACACCGCCCGTCGTTGCTACTGATTGGCTGTAGGGTAGAGATCGGC2030.24407252440725244No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAATCTTT1960.23565623046217476No Hit
GTACACACCGCCCGTCGTTTCTTCCGACTGATACTCATTGTGAGTTGCAA1940.23325157504929545No Hit
GTACACACCGCCCGTCGCACATACCGATTGAATGATTCGGTGAAAATCTC1900.22844226422353678No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAATCTTC1840.2212282979848988No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGCATTT1500.18034915596595008No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATCATTCGGTGAGGATCTC1380.16592122348867408No Hit
GTACACACCGCCCGTCGCACCTACCGATTGGATGGTCCGGTGAAGCCTCG1350.16231424036935507No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGGTCCGGTGAGGCCTTG1320.15870725725003607No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCTTTCT1300.15630260183715675No Hit
GTACACACCGCCCGTCGCACCTACCAATTGAATGATTAGGTGAAAATCTC1270.15269561871783774No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAACGTGCTGGTGAAGCGTTA1270.15269561871783774No Hit
GTACACACCGCCCGTCTCACCTACCGATTGAATGGTCCGGTGAGGCCTCG1170.14067234165344106No Hit
GTACACACCGCCCGTCGTTTCTTCCGACTGATATTCAATGTGAATTGTGA1120.13466070312124273No Hit
GTACACACCGCCCGTCTCACCTACCGATTGAATGATTCGGTGAAAATCTC1100.13225604770836338No Hit
GTACACACCGCCCGTCGCACCAACCGATTGAATGGTTCGGTGAAGAATCT1100.13225604770836338No Hit
GTACACACCGCCCGTCGCACCAACTGATTGGATGACTCGGTGAAAAATTG1090.13105372000192372No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGACTCG1090.13105372000192372No Hit
GGTACTGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC1070.1286490645890444No Hit
GGTACAGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC1030.12383975376328574No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGTCATAGTGAGGTCCTC1010.12143509835040639No Hit
GGTACTGGTTGAACTGTTTATCCCCCATTGTCTAGTATCACAGCACACTC1000.12023277064396672No Hit
GGAACTGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC1000.12023277064396672No Hit
GGAACTGGTTGAACTGTTTATCCCCCATTGTCTAGTATCACAGCACACTC960.11542345981820805No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAGTGGTCCGGTGAGATCTCG920.1106141489924494No Hit
GGTACTGGATGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC910.10941182128600972No Hit
GGTACTGGTTGAACAGTTTATCCTCCATTGTCTAGTATCACAGCACACTC900.10820949357957005No Hit
GGTACAGGTTGAACTGTTTATCCCCCATTGTCTAGTATCACAGCACACTC900.10820949357957005No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGGGTGATCCGGTTAATTCCAC880.1058048381666907No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATCTTC880.1058048381666907No Hit
GTACACACCGCCCGTCGCTACCACCGATTGAATGGCTTAGTGAGATTTCC870.10460251046025104No Hit
GGTACTGGTTGAACTGTATATCCCCCATTGTCTAGTATCACAGCACACTC860.10340018275381138No Hit
GTACACACCGCCCGTCGCTACTACCGATTTAATGTTTTAGTGAGGTCCTC850.10219785504737172No Hit
GGAACAGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC850.10219785504737172No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)