FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5u_S126_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5u_S126_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86986
Total Bases26.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1673919.243326512312326No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1290314.83342147012163No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1055912.138734968845561No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC28533.279838134872278No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC20062.3061182259214124No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC17101.965833582415561No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC15451.7761478858666913No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC8220.9449796518980066No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC6470.743797852527993No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC5580.6414825374198148No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC4940.5679074793644955No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC3790.43570229692134366No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC3730.4288046352286575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3650.4196077529717426No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG2480.2851033499643621No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG2320.2667095854505323No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2150.2471662106545881No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG1960.22532361529441516No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1770.2034810199342423No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG1770.2034810199342423No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG1730.19888257880578483No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1620.18623686570252684No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1620.18623686570252684No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG1620.18623686570252684No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG1450.16669349090658267No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1450.16669349090658267No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA1440.1655438806244683No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1300.1494493366748672No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1290.14829972639275285No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAGTGTTGATAGAGAATAGG1260.14485089554640976No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAGTGTTGATAGAGAATAGG1200.1379532338537236No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAGTGTTGATAGAGAATAGG1200.1379532338537236No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAATAAGTGTTGATAGAGAATAGG1190.13680362357160922No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG1130.12990596187892306No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1110.12760674131469432No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1100.12645713103257994No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAGTGTTGATAGAGAATAGG1100.12645713103257994No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1090.1253075207504656No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAGTGTTGATAGAGAATAGG1060.12185868990412249No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1060.12185868990412249No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTCTCCTTC1040.11955946933989378No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1000.11496102821143632No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG930.10691375623663579No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG910.10461453567240706No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC900.1034649253902927No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG900.1034649253902927No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAATAAGTGTTGATAGAGAATAGG890.10231531510817833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)