FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5t_S125_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5t_S125_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339086
Total Bases102 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTCG4923314.519325480851467No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACATTTTAGTGAGGTCCTC321709.487268716490801No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGTCATAGTGAGGTCCTC280398.268993706611303No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTATTT265077.817190919117864No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTCCTC95862.8270114366266963No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGCCTCG91452.6969559344826974No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTGAGTGGTCCGGTGAATCTTT85132.5105725391198694No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTCGAGTGGTCCGGTGAATCTT28210.8319423391116119No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGCATTT28180.8310576078045098No Hit
GTACACACCGCCCGTCGCTCCTACCGATCGAGTGTTTAGGTGAATTTGAT26370.7776788189426871No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATTTACTAGTGAGACAATA19750.5824481105088385No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCTCTCT17170.5063612180980637No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGAATTAGTGAGGCCTTT13200.3892817751248946No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTCCAC11790.34769940369109903No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTC10760.3173236288139292No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGACTCG7950.23445379638203878No Hit
GTACACACCGCCCGTCGCACCAACCGATTGAATGGTTTGGTGAAATATTG7500.22118282677550827No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGATCCTC7450.21970827459700487No Hit
GTACACACCGCCCGTCGCAGCTACTGATTGAATACATCGGTGAAATATTG7060.20820676760467843No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTTG6980.20584748411907303No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAGTGTTTTAGTGAGGACCTC5900.17399715706339985No Hit
GTACACACCGCCCGTCGTTGCTACCGATTGGCTGTGGGATAGAGCTGGAC5640.16632948573518222No Hit
GTACACACCGCCCGTCGTTTCTTCCGACTGATACTCATTGTGAGTTGCAA5580.16456002312097817No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAATCTTC5410.15954654571406662No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGTTTCGGTGAAGATTCC4780.14096718826492394No Hit
GTACACACCGCCCGTCGCTACTACCGATGGATGATTTGGTGAGGTCGCCT4390.12946568127259753No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATCCTC3980.11737435340886972No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAACGTGCTGGTGAAGACTTC3820.11265578643765888No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTCCGGTGAGGCCTCG3710.10941177164495142No Hit
GTACACACCGCCCGTCGCACCTACCGATTGGATGGTCCGGTGAGAATTTC3450.10174410031673381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)