FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5s_S124_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5s_S124_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133845
Total Bases40.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2571319.211027681273116No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1817613.579887182935485No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1423010.631700847995816No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC51793.8694011729986175No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC36542.730023534685644No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC26651.991109118756771No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC14261.0654114834323285No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC10770.8046621091561134No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC9940.7426500803167844No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC7560.5648324554522022No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACAACTTCACCTTC5800.43333706899772123No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC5740.42885427173222757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4720.3526467182188352No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC3610.26971496880720236No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG3120.23310545780567074No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3010.22488699615226568No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2870.21442713586611378No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2780.2077029399678733No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG2450.1830475550076581No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAACAAGTGCTGGTAAAGAACAGG2240.16735776457843027No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG2040.1524151070267847No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2030.15166797414920244No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG1950.1456909111285442No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG1900.1419552467406328No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1870.139713848107886No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG1820.13597818371997458No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG1750.13074825357689865No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAACAAGTGCTGGTAAAGAACAGG1750.13074825357689865No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG1730.12925398782173408No Hit
TGATCCTTCTGCAGGTTCACCTACGGTAACCTTGTTACGACTTCACCTTC1720.12850685494415182No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG1690.12626545631140498No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG1640.1225297919234936No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1590.1187941275355822No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG1580.11804699465799992No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA1580.11804699465799992No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAACAAGTGCTGGTAAAGAACAGG1580.11804699465799992No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG1570.11729986178041765No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGATGTTGATATAAGATAGG1570.11729986178041765No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAACAAGTGCTGGTAAAGAACAGG1550.1158055960252531No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTGTCCTTC1540.11505846314767082No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAACAAGTGCTGGTAAAGAACAGG1500.1120699316373417No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC1480.11057566588217715No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG1460.1090814001270126No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG1460.1090814001270126No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG1440.10758713437184803No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTCCACCTTC1440.10758713437184803No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAAAAGATGTTGATATAAGATAGG1420.10609286861668348No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG1420.10609286861668348No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG1410.1053457357391012No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC1370.10235720422877208No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAACAAGTGCTGGTAAAGAACAGG1360.10161007135118981No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAACAAGTGCTGGTAAAGAACAGG1350.10086293847360753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)