FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5q_S122_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5q_S122_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158574
Total Bases47.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7250.4571997931565074No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC4730.29828345125934896No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4490.2831485615548577No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG4330.27305863508519684No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAAAACAGG3310.2087353538411089No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2700.17026750917552688No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC2660.1677450275581117No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2530.15954696230151222No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG2520.15891634189715842No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGATATAAAATTGG2320.14630393381008236No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2220.13999772976654432No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAAAACAGG2170.13684462774477532No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2160.1362140073404215No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAAAACAGG2100.1324302849142987No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGCTGGTACAGCACTGG2030.12801594208382208No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2000.12612408087076066No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG1890.11918725642286881No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1820.1147729135923922No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG1800.1135116727836846No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAAAACAGG1760.11098919116626937No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGATATAAAATTGG1680.10594422793143894No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGATATAAAATAGG1670.10531360752708514No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1670.10531360752708514No Hit
TAGACCTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1630.10279112590966992No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1620.10216050550531613No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAAAACAGG1610.10152988510096232No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAATAAATGCTGATATAAAATCGG1590.10026864429225472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)