FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5o_S116_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5o_S116_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110248
Total Bases33.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC2575423.360060953486684No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2114619.180393295116467No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC57045.173790000725637No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC45764.150642188520426No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC38453.487591611639213No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC10990.996843480153835No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC9780.8870909222843044No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC8680.7873158696756404No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC5190.47075683912633337No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4700.4263115884188375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4560.41361294535955295No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA3080.27937014730425946No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC3010.2730208257746172No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2450.22222625353747913No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC2300.2086205645453886No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG2080.18866555402365576No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC2060.18685146215804369No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1980.1795950946955954No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG1920.17415281909875915No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG1870.16961758943472896No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1750.15873303824105653No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC1730.15691894637544446No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG1640.14875553298019012No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG1630.14784848704738407No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG1620.14694144111457805No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1570.14240621145054785No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC1550.14059211958493578No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG1540.13968507365212973No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGCTGGTAAAGCACTGG1520.13787098178651766No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1490.13514984398809957No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAAAGATGCTGGTAAAGCACTGG1470.13333575212248747No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1460.13242870618968144No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC1430.12970756839126332No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1390.12607938466003918No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG1340.12154415499600899No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC1340.12154415499600899No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1310.11882301719759089No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG1310.11882301719759089No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGATGTTGGTAGAGTACTGG1290.11700892533197882No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG1280.11610187939917278No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAAAGATGTTGGTAGAGTACTGG1220.11065960380233655No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1220.11065960380233655No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1220.11065960380233655No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1190.10793846600391845No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGATGTTGGTAGAGTACTGG1170.10612437413830636No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAAAGATGCTGGTAAAGCACTGG1150.1043102822726943No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1150.1043102822726943No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGGTGTTGGTAGAGTACTGG1120.10158914447427618No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1120.10158914447427618No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC1110.10068209854147013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)