FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5n_S115_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5n_S115_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110863
Total Bases33.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2295020.70122583729468No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1804916.280454254349962No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1139710.280255811226468No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC43953.9643523989067586No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC35073.1633637913460757No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC20991.8933278009795873No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC11961.0788089804533523No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC11050.9967256884623363No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC8970.8091067353400143No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC7620.6873348186500455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7100.640430080369465No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTCTCCTTC5060.4564191840379568No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC4350.3923761760010102No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC4340.39147416180330674No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC4200.3788459630354582No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA3830.3454714377204297No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC2870.2588780747408964No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2600.23452369140290266No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACATTGTTACGACTTCTCCTTC2380.21467937905342632No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC2130.19212902411083949No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC1990.17950082534299092No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC1810.1632645697843284No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTAACCTTC1700.15334241360959022No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTCTCCTTC1660.14973435681877634No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1540.13891018644633465No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC1180.10643767532900969No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1170.10553566113130622No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1110.10012357594508538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)