FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5m_S110_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5m_S110_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122031
Total Bases36.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2816123.076923076923077No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC2440419.998197179405235No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1324610.854618908310183No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC37933.1082265981594843No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC32922.6976751808966575No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC16471.3496570543550408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4510.3695782219272152No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC4460.3654809023936541No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC3710.3040211093902369No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC3160.2589505945210643No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC3120.2556727388942154No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG2970.243380780293532No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG2790.22863042997271185No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC2620.21469954355860396No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2440.19994919323778385No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2200.18028205947669035No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2080.1704484925961436No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG2020.16553170915587023No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1890.15487867836861124No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG1880.15405921446189902No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1800.1475035032082012No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG1790.14668403930148896No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG1760.14422564758135228No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGCCTTATCCTTC1750.14340618367464006No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA1740.14258671976792783No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG1640.13439208070080555No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC1590.1302947611672444No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG1550.12701690554039546No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG1530.125377977726971No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1410.11554441084642426No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1350.1106276274061509No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1350.1106276274061509No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1340.10980816349943866No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG1330.10898869959272645No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG1330.10898869959272645No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1280.10489138005916529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)