FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5k_S108_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5k_S108_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62184
Total Bases18.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1461923.509262832883056No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC755512.149427505467644No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC657810.5782838029075No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC19343.110124790943008No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC9601.5438054805094559No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC9381.5084266049144475No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC8611.3846005403319182No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC6311.0147304772931944No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC5320.8555255371156567No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC5130.8249710536472405No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC3920.6303872378746944No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC3170.5097774347098932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.43580342210214845No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2220.35700501736781165No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2020.3248424031905313No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC1610.25890904412710664No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG1560.2508683905827866No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG1330.21388138427891418No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC1330.21388138427891418No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG1300.20905699215232215No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG1210.194583815772546No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG1200.19297568506368198No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG1170.18815129293708993No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC1160.1865431622282259No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG1110.17850250868390583No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAAAAGATGTTGATATAAGATAGG1070.17206998584844976No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG1030.1656374630129937No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTCTCCTTC1000.16081307088640165No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG970.1559886787598096No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG950.15277241734208158No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTGTCCTTC940.15116428663321754No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG940.15116428663321754No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG900.14473176379776148No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTGCTTC790.1270423260002573No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAAAAGATGTTGATATAAGATAGG790.1270423260002573No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGATGTTGATATAAGATAGG780.1254341952913933No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG760.12221793387366525No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA730.11739354174707321No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAAAAGATGTTGATATAAGATAGG700.11256914962048115No Hit
TAGACCTCGGGGTGGCCGAAAAACCAAAAAAGATGTTGATATAAGATAGG680.10935288820275312No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG670.1077447574938891No Hit
TAGACCTCGGGATGGCCAAAAAATCAAAAAAGATGTTGATATAAGATAGG650.10452849607616108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)