FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5h_S98_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5h_S98_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377307
Total Bases113.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC60

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC19081050.5715504880641No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC6697517.750797096263785No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC286877.603092441963706No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC229106.071978521469255No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC100142.654072148144614No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC32590.8637528590776211No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC32390.8584521357939291No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC13710.3633645810970907No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC11960.3169832523647852No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC11850.3140678545587545No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC9970.2642410556920492No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC8120.2152093653178976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8020.21255900367605157No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC6010.1592867346749464No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC5790.15345593906288513No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAGCCTTGTTACGACTTTTACTTC5560.14736010728663926No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA5310.14073420318202418No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACCTC5150.13649362455507053No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACCTTTACTTC4880.12933764812208626No Hit
TGATCCTTCTGCAGGTTCACCTCCGGAAACCTTGTTACGACTTTTACTTC4760.12615721415187103No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC4750.12589217798768643No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTATACTTC4450.11794109306214834No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC4380.1160858399128561No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTTTACTTC4230.11211029745008708No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGGCTTTTACTTC3980.105484393345472No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC3950.10468928485291819No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGCTACGACTTTTACTTC3830.10150885088270295No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTACTTC3790.10044870622596452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)