FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5ff_S137_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5ff_S137_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424471
Total Bases127.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC6818016.062345837524823No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC6176214.550346195617603No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC383739.04019355857055No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC120572.840476734570795No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC110942.6136061120783283No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC64751.5254281211201708No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC55811.3148130260960111No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC55581.3093945169399088No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC38280.9018283934591528No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC37030.8723799741325086No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC31480.741628992322208No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC21930.5166430686666463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19180.45185654614802895No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC15380.36233335139503053No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC15090.3555013181112491No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC14740.34725576069978864No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTT10720.25254964414530084No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG10040.2365297040316064No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9610.22639944778324078No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC9360.22050976391791194No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG8290.1953019169743045No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC7730.18210902511596788No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC7610.17928197686061004No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG7120.16773819648456548No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG7080.1667958470661129No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG7030.1656179102930471No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG6730.15855028965465248No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA6690.15760794023619987No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTGACGACTTAACCTAC6210.14629974721476852No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG6030.14205917483173172No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC6020.14182358747711857No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACTACTTTTACTTC5930.13970330128560018No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG5830.13734742773946867No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAGAGATGTTGGAATAGGACCGG5770.13593390361178972No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG5460.128630695618782No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCTTC5450.1283951082641688No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG5430.1279239335549425No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG5420.12768834620032937No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAGAGATGTTGGAATAGGACCGG5350.12603923471803727No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG5300.1248612979449715No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG5090.11991396349809527No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG4900.11543780376044535No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC4850.11425986698737958No Hit
TGATCCTTCTGCAGGTTCACCTACATATACCTTGTTACGACTTAACCCTC4790.11284634285970066No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGATGCTGGTATAAAATAGG4780.11261075550508752No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG4540.10695665899437182No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG4520.10648548428514551No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC4480.1055431348666929No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAGAGATGTTGGAATAGGACCGG4420.10412961073901397No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAGAGATGTTGGAATAGGACCGG4340.10224491190210874No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG4320.10177373719288245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)