FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5f_S96_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5f_S96_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8751
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC293933.584733173351616No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC6507.427722545994743No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC5416.182150611358702No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC4074.650897040338247No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1201.3712718546451834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG961.0970174837161468No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC650.7427722545994744No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC550.628499600045709No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500.5713632727688265No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC430.49137241458119074No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC220.25139984001828364No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG200.22854530910753054No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC190.21711804365215404No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC180.2056907781967775No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG170.19426351274140097No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG150.17140898183064793No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC150.17140898183064793No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG150.17140898183064793No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC120.13712718546451835No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCTTC120.13712718546451835No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG120.13712718546451835No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG120.13712718546451835No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC120.13712718546451835No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG120.13712718546451835No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG110.12569992000914182No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTCTTACTTC110.12569992000914182No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG110.12569992000914182No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG110.12569992000914182No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAGCCTTGTTACGACTTTTACTTC100.11427265455376527No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAATAAATGTTGATATAAAATAGG100.11427265455376527No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG100.11427265455376527No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG100.11427265455376527No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG100.11427265455376527No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC90.10284538909838875No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGGTGCTGGAACAAAATAGG90.10284538909838875No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAATAAATGTTGATATAAAATAGG90.10284538909838875No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTCC90.10284538909838875No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG90.10284538909838875No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC90.10284538909838875No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGGTGCTGGAACAAAATAGG90.10284538909838875No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC90.10284538909838875No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG90.10284538909838875No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG90.10284538909838875No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG90.10284538909838875No Hit
TGATCCTTCTGCAGGTTCACCTCCGGAAACCTTGTTACGACTTTTACTTC90.10284538909838875No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG90.10284538909838875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)