FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5ee_S136_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5ee_S136_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322086
Total Bases96.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC8737827.128779270132817No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC6016418.67948311941531No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC3407210.578541134976373No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC165045.124097290785691No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC115953.599970194295933No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC60231.869997454096111No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC59841.8578888868190484No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC30570.9491253888712952No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC27530.8547406593270122No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTAGCTTC26070.8094111510590338No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC25050.7777425904882547No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC17760.5514055252323914No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC17680.5489217165601734No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC15780.4899312605949964No Hit
TGATCCTTCTTCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC11650.3617046378917432No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTAGCTTC11300.3508379749507895No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC10030.3114075122793291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8410.26111038666691505No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC7250.22509516091975437No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC6380.19808374160938383No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA5820.18069708090385797No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC5100.15834280285389615No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4750.1474761399129425No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC4520.14033518998031583No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4150.12884757487130766No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG4130.12822662270325316No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC3870.12015424451854474No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTGACGACTTAACCCTT3840.11922281626646299No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG3460.1074247250734276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)