FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5e_S95_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5e_S95_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349213
Total Bases105.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC17570650.3148508217048No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC4027611.533362160057042No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC303678.695838929249485No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC299998.590459118074069No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC68201.9529628049356695No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC50421.4438179563761944No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC33130.9487046587612717No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC24270.6949913090291598No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC15650.44815055567805323No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC13160.3768473682251233No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC11130.31871665716912023No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC10680.30583053895473533No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC7670.2196367260096274No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC7310.20932783143811942No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC7140.20445974233490738No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC5750.16465595496158505No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACCTTTACTTC5420.15520613493770277No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA5390.15434706039007712No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACCTC4790.1371655694375639No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4770.13659285307248012No Hit
TGATCCTTCTGCAGGTTCACCTCCGGAAACCTTGTTACGACTTTTACTTC4740.13573377852485444No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAGCCTTGTTACGACTTTTACTTC4730.13544742034231255No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTTTACTTC4480.1282884657787654No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4300.12313401849301142No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTACTTC4170.11941136211996689No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCTTC4160.11912500393742502No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC4150.11883864575488313No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGGCTTTTACTTC4050.11597506392946426No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC3840.10996154209608462No Hit
TGATCCTTCTGCAGGTTCACTACGGAAACCTTGTTACGACTTTTACTTCC3770.10795703481829141No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGCTACGACTTTTACTTC3630.10394802026270498No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTCGTTACGACTTTTACTTC3520.10079808025474425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)