FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5cc_S134_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5cc_S134_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428264
Total Bases128.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC10199423.81568378383427No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC8256219.278295630732444No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC269116.283740870117498No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC143693.355173444417462No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC115702.701604617712439No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC38440.8975771953748155No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC35900.8382679842340238No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC32900.7682177348551361No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC20990.4901182448209516No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC17740.4142304746604898No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC15720.367063306745372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14940.3488502419068612No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA14070.3285356695869837No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC13690.3196626379989913No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG13370.3121906113985766No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG11410.2664244484710366No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG11190.26128743018325146No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC9960.23256682793790745No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG9790.22859731380643714No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG9750.2276633104813853No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC9720.22696280798759644No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG9070.21178525395550407No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG9000.21015074813666335No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG8410.1963741990921488No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG7840.1830646517101601No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG7830.18283115087889712No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG7640.1783946350849009No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG7450.17395811929090468No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC7390.17255711430332693No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG7370.172090112640801No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG7100.1657855901967011No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG6960.16251657855901966No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG6920.16158257523396785No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG6730.15714605943997162No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6700.15644555694618273No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG6670.15574505445239384No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG6620.15457755029607906No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG6400.14944053200829394No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG6150.14360301122671998No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC6120.1429025087329311No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC5950.13893299460146077No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG5790.13519698130125343No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGATGTTGATATAAGATAGG5790.13519698130125343No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG5610.13099396633852015No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC5610.13099396633852015No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG5430.1267909513757869No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC5190.12118693142547587No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG5120.11955242560663516No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG5070.11838492145032037No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG4940.1153494106439019No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGATGCTGGTATAAAATAGG4830.11278090150000934No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAAAAGATGTTGATATAAGATAGG4820.11254740066874637No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAAAAGATGTTGATATAAGATAGG4610.10764388321222423No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAAAAGATGTTGATATAAGATAGG4590.10717688154969832No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG4320.10087235910559841No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAAAAGATGCTGGTATAAAATAGG4300.1004053574430725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)