FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5c_S89_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5c_S89_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61812
Total Bases18.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1538324.886753381220476No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC636710.300588882417653No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC61269.910696952048147No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC29424.7595936064194655No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC11271.823270562350353No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC11141.8022390474341552No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC9941.6081019866692552No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC5680.9189154209538601No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4490.7263961690286677No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC3320.53711253478289No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC2690.4351905778813175No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC2580.417394680644535No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTAT2410.38989193036950753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2120.34297547401799No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC1630.26370284087232254No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC1580.25561379667378503No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA1300.21031514916197502No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1020.16501650165016502No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG990.1601630751310425No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG940.15207403093250502No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTGTCCTTC920.14883841325309002No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG890.1439849867339675No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG880.14236717789426No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG850.13751375137513752No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG770.1245712806574775No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC770.1245712806574775No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG750.1213356629780625No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG730.11810004529864751No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG710.11486442761923252No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG700.11324661877952502No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG690.1116288099398175No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG690.1116288099398175No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG680.11001100110011No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG680.11001100110011No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC660.10677538342069501No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG650.10515757458098751No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG650.10515757458098751No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG650.10515757458098751No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG640.10353976574128002No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG620.100304148061865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)