FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5bb_S133_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5bb_S133_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381082
Total Bases114.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC8200921.520040306285786No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC6386316.75833547635417No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC200955.273143312987756No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC155334.0760256322786175No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC123783.2481198272287855No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC37520.9845650017581518No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC28850.7570549120661695No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC23620.619814108249668No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC16120.4230060721839395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15140.39728982213801756No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC12380.32486446486582937No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC11970.3141056255609029No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA10180.2671341076198824No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC9590.25165187544937834No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9580.25138946473462404No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG8680.22777250040673663No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG7720.20258107179032334No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC7530.19759526820999157No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG6730.17660241102964716No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG6650.17450312531161272No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG6640.17424071459685844No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC6640.17424071459685844No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG6090.15980812528537167No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG5900.15482232170503984No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG5770.1514109824132339No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG5630.14773723240667364No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG5510.14458830382962198No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC5350.1403897323935531No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAAAGATGTTGGTAGAGTACTGG5240.13750321453125575No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC5190.1361911609574842No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC4940.1296308930886266No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG4880.12805642880010076No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG4870.12779401808534646No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG4850.12726919665583786No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG4830.12674437522632925No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG4630.12149616093124316No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC4570.11992169664271732No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG4560.11965928592796302No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGATGTTGGTAGAGTACTGG4440.11651035735091135No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC4430.11624794663615706No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG4340.11388625020336832No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGATGTTGGTAGAGTACTGG4230.11099973234107097No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG4200.11021250019680805No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC4180.10968767876729942No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG4130.1083756251935279No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG4130.1083756251935279No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAAAGATGTTGGTAGAGTACTGG4090.10732598233451067No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGCTGGTAAAGCACTGG4060.10653875019024776No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG4030.10575151804598487No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG4030.10575151804598487No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGATGTTGGTAGAGTACTGG3990.10470187518696764No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG3970.10417705375745903No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG3970.10417705375745903No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAAAGATGTTGGTAGAGTACTGG3950.10365223232795041No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGGTGTTGGTAGAGTACTGG3890.10207776803942457No Hit
TAGACCTCGGGGTGGCCAAAAAATCAGAAAAGATGTTGGTAGAGTACTGG3850.10102812518040737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)