FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5b_S88_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5b_S88_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203100
Total Bases61.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC6023529.65780403741999No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC3262916.06548498276711No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC106175.227474150664698No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC97364.79369768586903No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC47052.3165928114229444No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC16100.7927129492860661No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC14610.7193500738552437No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11720.5770556376169375No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC7840.3860167405219104No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC7530.3707533234859675No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC6410.3156080748399803No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG5700.2806499261447563No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC5690.2801575578532743No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTCTCCTTC5640.2776957163958641No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG5290.2604628261939931No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTCC4870.2397833579517479No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4570.22501230920728704No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG4520.2225504677498769No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG4240.2087641555883801No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG4090.20137863121614968No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG3890.1915312653865091No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG3610.1777449532250123No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGCTGGTATAAAATAGG3540.17429837518463812No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG3400.1674052191038897No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG3180.1565731166912851No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGATGCTGGTATAAAATAGG2880.1418020679468242No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG2880.1418020679468242No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG2840.1398325947808961No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2760.13589364844903987No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG2670.1314623338257016No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2510.12358444116198916No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGCTGGTATAAAATAGG2460.12112259970457903No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG2400.11816838995568685No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC2380.11718365337272278No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAAAAGATGCTGGTATAAAATAGG2350.11570654849827672No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2350.11570654849827672No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGATGTTGATATAAGATAGG2310.1137370753323486No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAAAAGATGCTGGTATAAAATAGG2260.11127523387493846No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAAAAGATGCTGGTATAAAATAGG2250.11078286558345643No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAAAAGATGCTGGTATAAAATAGG2230.10979812900049238No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGATGTTGATATAAGATAGG2170.10684391925160021No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC2170.10684391925160021No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTCTTGTTT2120.10438207779419005No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC2100.103397341211226No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAAAAGATGCTGGTATAAAATAGG2060.10142786804529788No Hit
TAGACCTCGGGGTGGCCGAAAAACCAAAAAAGATGCTGGTATAAAATAGG2060.10142786804529788No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGATGTTGATATAAGATAGG2050.10093549975381585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)