FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5aa_S132_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5aa_S132_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312141
Total Bases93.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC6680521.402186832232868No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC6418220.56186146645266No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC196846.306124475797796No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC114823.6784658215357804No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC114093.655078954703163No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC33321.067466305291519No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC32731.0485645910021433No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC28620.9168933270541197No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC17990.5763421018065553No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA14120.4523596707898033No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC13900.44531157393613785No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC13270.42512838749155024No Hit
TGATCCTTCTGCAGGTTCACCTACGATAACCTTGTTACGACTTTTTCTTC13090.41936176279309667No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC10330.3309401840834751No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10320.3306198160446721No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC7860.2518092784991398No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC6870.2200928426576451No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC6070.19446339955340697No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC5780.18517272642812063No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC5710.18293015015649977No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGTTTC5620.18004683780727299No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCAGCTTC5210.16691174821635094No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG5160.16530990802233606No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4930.1579414431298676No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG4590.1470489298105664No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC4570.14640819373296043No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC4020.1287879515987967No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG4000.12814721552119074No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTTTTTC3810.12206022278393419No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAATAAATGTTGGTATAAAACTGG3730.11949727847351035No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG3670.11757507024069251No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG3630.11629359808548059No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG3630.11629359808548059No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAAAGATGTTGGTAGAGTACTGG3590.1150121259302687No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG3440.11020660534822405No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAATAAATGTTGGTATAAAACTGG3370.10796402907660321No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGGTAGAGTACTGG3340.10700292496019427No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG3250.10411961261096749No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG3200.10251777241695259No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCCCG3190.10219740437814961No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAATAAATGTTGGTATAAAACTGG3140.10059556418413475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)