FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5aa_S132_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5aa_S132_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312141
Total Bases93.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGCCTCG4639714.864115896341717No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGATCCTC3367210.787432602573837No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTCG159655.114675739489526No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGTCATAGTGAGGTCCTC98033.1405678843855824No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTCCTC44781.4346080777597303No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCTCTCT40221.288520252065573No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTCGAGTGGTCCGGTGAATCTT38601.2366206297794906No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACATTTTAGTGAGGTCCTC36141.1578100922339585No Hit
GTACACACCGCCCGTCGCAGCTACTGATTGAATACATCGGTGAAATATTG31851.0203722035874814No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGACTCG29760.9534152834776592No Hit
GTACACACCGCCCGTCGCTCCTACCGATCGAGTGTTTAGGTGAATTTGAT25810.8268699081504833No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTGAGTGGTCCGGTGAATCTTT23500.7528648911869956No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGCATTT20450.6551526393520877No Hit
GTACACACCGCCCGTCGCACCAACCGATTGAATGGTTTGGTGAAATATTG20280.6497063826924371No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTC20200.6471434383820133No Hit
GTACACACCGCCCGTCGCTACTACCGATCGGATGGTTTAGTGAGACCCTC19690.6308046684030615No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGTTTCGGTGAAGATTCC14800.47414469742840576No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGTTTTAGTGAGGTATTT12760.40878961751259846No Hit
GTACACACCGCCCGTCGCACCAACTGATTGGATGACTCGGTGAAAAATTG12150.38924716714561686No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATGTTC11820.37867502186511864No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGACGTTTTAGTGAGACATTT11800.3780342857875127No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTTG11330.36297698796377276No Hit
GTACACACCGCCCGTCAAACCATCTTAGTTGGGGGTGGGTGAGGCTGCGC10850.3475993221012299No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGGTTTAGTGAGGGCCTC10590.33926975309235247No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGCTTAGTGAGCTTTCT9790.3136403099881144No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAACGTGCTGGTGAAGCGTTA9670.3097958935224786No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATCCTC9170.29377749158232974No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAATCTTC8840.28320534630183153No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGACCCAC8470.2713517288661214No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAATCTTC7780.249246334188716No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGACTCG7570.24251860537385347No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAATCTTT7240.23194646009335523No Hit
GTACACACCGCCCGTCGCTGTTACCGATTGAGTGTTAAGGTGAACCTTCT6600.21144290560996473No Hit
GTACACACCGCCCGTCGCACCAACCGATTGAATGGTTCGGTGAAGAATCT5980.19158008720418016No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATCATTCGGTGAGGATCTC5820.18645419858333254No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAGTGGTCCGGTGAACCCTCG5750.1842116223117117No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGGTCCGGTGAGGCCTTG5730.18357088623410575No Hit
GTACACACCGCCCGTCGTTTCTTCCGACTGATACTCATTGTGAGTTGCAA5660.18132830996248492No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATCATTCGGTGAGGATTCC5620.18004683780727299No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGCGTTC5550.17780426153565218No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGTCTTC5400.1729987409536075No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGATGAATATCGT5050.16178585959550332No Hit
GTACACACCGCCCGTCGCTACGACCGATTGTTCCAGTTAATGAGCGATAT4980.15954328332388248No Hit
GGTACTGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC4730.15153408235380805No Hit
GTACACACCGCCCGTCGCTTTTACCGATTGAGTGTTCAGGTGAACCTTCT4640.14865077000458124No Hit
GTACACACCGCCCGTCGTTGCTACCGATTGGCTGTGGGATAGAGCTGGAC4610.14768966588817234No Hit
GGTACAGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC4380.14032120099570386No Hit
GGTACTGGTTGAACTGTTTATCCCCCATTGTCTAGTATCACAGCACACTC4320.138398992762886No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAGTGATTTAGTGAGGTCTTG4260.13647678453006815No Hit
GTACACACCGCCCGTCTCACCTACCGATTGAATGGTCCGGTGAAGCCTCG4190.13423420825844729No Hit
GGTACTGGTTGAACAGTTTATCCTCCATTGTCTAGTATCACAGCACACTC4190.13423420825844729No Hit
GGAACTGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC4130.13231200002562946No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGGCTTAGTGAAATGTGA4130.13231200002562946No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGGTCCGGTGAATTAACC4070.13038979179281157No Hit
GGTACAGGTTGAACTGTTTATCCCCCATTGTCTAGTATCACAGCACACTC3880.12430279905555502No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGTTCCTC3800.12173985474513122No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGCTCAGGTGAGCCATTC3750.12013801455111632No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAATGTGTTGGTGAGGAGCTC3740.11981764651231334No Hit
GGTACTGGTTGAACTGTATATCCCCCATTGTCTAGTATCACAGCACACTC3630.11629359808548059No Hit
GTACACACCGCCCGTCGCACCTACCGATTGGATGGTCCGGTGAGAATTTC3600.11533249396907168No Hit
GTACACACCGCCCGTCACACCATGGGAGTTGGTTCTACCCGACGACGCTG3570.11437138985266274No Hit
GGTACTGGTTGAACTGTTTACCCTCCATTGTCTAGTATCACAGCACACTC3560.11405102181385976No Hit
GGTACTGGATGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC3510.11244918161984487No Hit
GGAACAGGTTGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC3500.11212881358104189No Hit
GGAACTGGATGAACTGTTTATCCTCCATTGTCTAGTATCACAGCACACTC3470.11116770946463297No Hit
GGTACTGGTTGAACTGTTTACCCCCCATTGTCTAGTATCACAGCACACTC3450.11052697338702702No Hit
GTACACACCGCCCGTCGCACCTACCGATTGGATGGTCCGGTGAAGCCTCG3450.11052697338702702No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACGGCTTAGTGAGTTGTAG3370.10796402907660321No Hit
GGAACTGGTTGAACTGTTTATCCCCCATTGTCTAGTATCACAGCACACTC3330.1066825569213913No Hit
GGTACTGGTTGAACAGTTTATCCCCCATTGTCTAGTATCACAGCACACTC3300.10572145280498237No Hit
GGTACAGGTTGAACAGTTTATCCTCCATTGTCTAGTATCACAGCACACTC3290.10540108476617938No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGTTCCGGTGAATAATTC3280.1050807167273764No Hit
GGTACTGGATGAACAGTTTATCCTCCATTGTCTAGTATCACAGCACACTC3270.10476034868857342No Hit
GGTACTGGTTGAACTGTATATCCTCCATTGTCTAGTATCACAGCACACTC3250.10411961261096749No Hit
GTACACACCGCCCGTCGCTGCCCGGAACTGAGCCGTTTCGAGAAATGTGG3220.10315850849455856No Hit
GGTACAGGTTGAACTGTATATCCTCCATTGTCTAGTATCACAGCACACTC3190.10219740437814961No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)