FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site5L_S109_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site5L_S109_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187718
Total Bases56.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC3746319.957063254456152No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC3306317.613121810375137No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC183369.767843254243067No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC90454.818397809480178No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC83394.442301750498088No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC41222.19584696193226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5920.3153666670218093No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC4300.22906700476246286No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4280.2280015768333351No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4200.22373986511682414No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC3900.20775844617990816No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC3680.19603873895950308No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC3510.18698260156191734No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2830.15075805197157438No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2550.1358420609637861No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2490.1326457771764029No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC2460.13104763528271132No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2410.12838406545989195No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG2210.11772978616861461No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2170.11559893031035916No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2160.1150662163457953No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2130.11346807445210369No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGATGTTGATATAAGATAGG1900.10121565326713475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)