FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1y_S120_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1y_S120_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157509
Total Bases47.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC4064725.80614441079557No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC154279.794360957151655No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC123817.860503209340419No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC80395.103835336393476No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC46392.9452285266238754No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC33122.1027369864579164No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC24731.5700690119294771No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC11610.7371007371007371No Hit
TGATCCTTCTTCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC9090.5771098794354609No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC8980.5701261515215004No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC6910.4387050898678806No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC6720.4266422871074034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5630.35743989232361323No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC5480.3479166269863944No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG3890.24697001441187486No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3860.2450653613444311No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3660.23236767422813934No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC3600.2285583680932518No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATT3590.22792348373743723No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC3580.22728859938162266No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG3360.2133211435537017No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3260.2069722999955558No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG3250.20633741563974123No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG3060.19427461287926404No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2830.17967227269552852No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2790.17713273527227016No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA2640.16760946993505133No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2640.16760946993505133No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC2540.16126062637690544No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2410.1530071297513158No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG2370.15046759232805745No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2310.14665828619316992No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2260.14348386441409697No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC2140.1358652521443219No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2110.13396059907687816No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG2110.13396059907687816No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2090.13269083036524898No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG2090.13269083036524898No Hit
TGATCCTTCTGCAGGTTCACCTACGGTTACCTTGTTACGACTTCACCCCA2060.1307861772978052No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2040.12951640858617602No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1990.1263419868071031No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG1980.1257071024512885No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG1970.12507221809547392No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG1970.12507221809547392No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1940.12316756502803014No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG1890.1199931432489572No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1860.11808849018151343No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC1850.11745360582569886No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1790.11364429969081133No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC1780.11300941533499674No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG1750.11110476226755296No Hit
TAGACCTCGGTGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1740.11046987791173837No Hit
TAGACTTCGGTGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG1720.10920010920010921No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG1720.10920010920010921No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1670.10602568742103625No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1670.10602568742103625No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1670.10602568742103625No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1640.10412103435359249No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTAT1630.1034861499977779No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG1630.1034861499977779No Hit
TAGACTTCGGTGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1600.10158149693033415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)