FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1w_S118_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1w_S118_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199881
Total Bases60.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC4927224.650667146952436No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC3498717.503914829323445No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2263611.324738219240448No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC92354.62024904818367No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC62063.104847384193595No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC43532.177795788494154No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC34361.7190228185770533No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC23661.1837043040609163No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC17410.8710182558622379No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC13640.6824060315887953No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC10050.5027991655034746No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC9790.4897914258984095No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC6570.32869557386645054No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC5350.26765925725806855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5170.2586538990699466No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC4930.24664675481911738No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC4140.20712323832680446No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC3850.19261460569038577No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA3850.19261460569038577No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC3640.18210835447091017No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG3210.16059555435484113No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC2920.14608692171842247No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2770.1385824565616542No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2300.11506846573711357No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG2260.11306727502864203No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTGCTTC2260.11306727502864203No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2020.1010601307778128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)