FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1r_S111_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1r_S111_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168168
Total Bases50.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC5113730.408282193996477No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC157039.337686123400408No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC144848.612815755672898No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC113996.778340706912135No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC33221.975405546834118No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC30761.8291232576946863No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC10940.6505399362542219No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC9660.5744255744255744No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC9360.5565862708719851No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC7930.471552257266543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5070.3014842300556586No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC4960.29494315208600924No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG4230.2515341801056087No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG4080.24261452832881406No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3580.21288235573949857No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG3420.20336806051091766No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG3010.178987678987679No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG2920.17363588792160223No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG2810.1670948099519528No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC2800.1665001665001665No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG2760.16412159269302126No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC2760.16412159269302126No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAGTGTTGATAGAGAATAGG2610.15520194091622663No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAGTGTTGATAGAGAATAGG2480.14747157604300462No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG2390.14211978497692784No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG2340.13914656771799627No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG2300.13676799391085104No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2080.12368583797155226No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAGTGTTGATAGAGAATAGG2050.12190190761619334No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC1920.11417154274297131No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAATAAGTGTTGATAGAGAATAGG1920.11417154274297131No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1890.1123876123876124No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAATAAGTGTTGATAGAGAATAGG1820.10822510822510822No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG1800.10703582132153562No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1790.10644117786974928No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG1730.10287331715903145No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1720.10227867370724514No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)