FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1p_S105_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1p_S105_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071180
Total Bases322.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC17666716.492746317145578No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC16274515.193058122817826No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC470444.391792229130491No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC318072.9693422207285423No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC296892.771616348326145No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC80460.7511342631490505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63270.5906570324315241No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC60710.5667581545585243No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC53120.4959017158647473No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC50430.4707892230997592No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35040.3271158908866857No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC33050.30853824754009596No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC29170.27231651076383057No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTATAAAATAGG26890.25103157265819004No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGGTGTTGGTATAAAATAGG21990.20528762672940123No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC21400.1997796822196083No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTATAAAATAGG21130.1972590974439403No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTATAAAATAGG19360.18073526391456152No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTATAAAATAGG19190.1791482290558076No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC18340.17121305476203813No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAAGAGGTGTTGGTATAAAATAGG16980.15851677589200697No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC16800.15683638604156164No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAGAGGTGTTGGTATAAAATAGG15690.1464739819638156No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAAGAGGTGTTGGTATAAAATAGG14920.13928564760357737No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTATAAAATAGG14780.1379786777198977No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTATAAAATAGG14500.13536473795253834No Hit
TAGACTTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTATAAAATAGG13610.12705614369200322No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG13490.12593588379170634No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC13340.12453555891633525No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAAGAGGTGTTGGTATAAAATAGG13320.12434884893295245No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC12880.12024122929853058No Hit
TAGACCTCGGGATGGCCGAAAAATCAGAAGAGGTGTTGGTATAAAATAGG12780.11930767938161653No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAGAGGTGTTGGTATAAAATAGG12530.11697380458933139No Hit
TAGACTTCGGGATGGCCGAAGAACCAGAAGAGGTGTTGGTATAAAATAGG11210.10465094568606584No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAAGAGGTGTTGGTATAAAATAGG11200.10455759069437442No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAGAGGTGTTGGTATAAAATAGG11140.10399746074422599No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG10820.10101010101010101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)